The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas

doi:10.1016/j.dci.2018.09.021

IOCAS-IR > 实验海洋生物学重点实验室

	The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas
	Wang, Lingling 1; Zhang, Huan 2; Wang, Mengqiang3 ; Zhou, Zhi 3; Wang, Weilin1 ; Liu, Rui 3; Huang, Mengmeng3 ; Yang, Chuanyan 1; Qiu, Limei 3; Song, Linsheng 1,3
	2019-02-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	91 页码:1-7
通讯作者	Zhang, Huan(zhanghuan@qdio.ac.cn) ; Song, Linsheng(lshsong@dlou.edu.cn)
摘要	Pattern recognition receptors (PRRs) are essential in recognizing specific pathogen-associated molecular patterns (PAMPs) on microbes and triggering responses to eliminate the invading pathogens. Previous genomic studies have revealed a great number of PRR genes in the Pacific oyster Crassostrea gigas, a sessile and filter-feeder marine bivalve belonging to the phylum Mollusca. On the survey of PRRs in the assembly oyster reference genome version 9, a total of 1084 PRRs were identified, which were composed of at least 12 gene families. Some of the gene families were significantly expanded, including C-type lectins (CTLs), fibrinogen-related proteins (FREPs), scavenger receptor cysteine-rich repeat protein (SRCRs), leucine-rich repeat (LRR)-only proteins (LRRops), and especially Clq domain-containing proteins (ClqDCs). The transcriptomic profiles of these abundant PRRs in response to PAMP treatments were investigated by RNA-Seq using the SOLiD EZ BeadTM system. Compared to the control library, there were 6,655, 7,273, 7,593, 6,830, 6687 and 8250 differentially expressed genes in the haemocytes of oysters in response to lipopolysaccharide (LPS) stimulation for 6 h, 12 h and 24 h, and peptidoglycan (PGN), glucan (GLU) and poly I:C (IC) stimulation for 12 h, respectively. After stimulation for 12 h, there were 134, 97, 114 and 159 genes up-regulated in the LPS, PGN, GLU and IC library, respectively. Most of the gene families involved in immune response towards PAMPs were ClqDCs, CTLs and FREPs, while only a few members of LRR and immunoglobin-containing proteins (LRRIGs), retinoic acid-inducible gene I [RIG-I]-like receptors (RLRs) and Toll like receptors (TLRs) were up-regulated. After LPS stimulation, the expression level of 258 non-redundant PRR genes in oyster haemocytes increased significantly with different expression pattern, and most of them were ClqDCs, CTLs, LRRops and FREPs. The transcriptomic analyses indicated that there was a dynamic and orchestrated specific expression regulation of numerous PRR genes in response to pathogen invasion. The expanded PRR gene family members were differentiated with more specific functional responses to certain PAMPs rather than the versatile ones. Based on the different expression pattern during the LPS stimulation, the oyster PRRs could be assigned into three consecutive steps in the response against pathogen invading. All the results would provide useful information for future studies of oyster PRRs and deep insight into the researches on invertebrate innate immunity.
关键词	Bivalve immunity Pattern recognition receptors Crassostrea gigas Transcriptome RNA-Seq
DOI	10.1016/j.dci.2018.09.021
收录类别	SCI
语种	英语
资助项目	National Science Foundation of China[U1706204] ; National Science Foundation of China[41476145] ; Modern Agro-industry Technology Research System[CARS-49] ; Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture, Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning ; Dalian Ocean University ; National Science Foundation of China[U1706204] ; National Science Foundation of China[41476145] ; Modern Agro-industry Technology Research System[CARS-49] ; Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture, Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning ; Dalian Ocean University
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000451492900001
出版者	ELSEVIER SCI LTD
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/156145
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Huan; Song, Linsheng
作者单位	1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
通讯作者单位	深海极端环境与生命过程研究中心; 实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Lingling,Zhang, Huan,Wang, Mengqiang,et al. The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,91:1-7.
APA	Wang, Lingling.,Zhang, Huan.,Wang, Mengqiang.,Zhou, Zhi.,Wang, Weilin.,...&Song, Linsheng.(2019).The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,91,1-7.
MLA	Wang, Lingling,et al."The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 91(2019):1-7.

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