Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus) | |
Yang, Yang1,2,3,5 ; Liu, Qinghua1,2,5; Xiao, Yongshuang1,2,5; Xu, Shihong1,2,5; Wang, Xueying1,2,5; Yang, Jingkun2; Song, Zongcheng4; You, Feng1,2,5; Li, Jun1,2,5
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| 2019-04-01 | |
| 发表期刊 | GENERAL AND COMPARATIVE ENDOCRINOLOGY
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| ISSN | 0016-6480 |
| 卷号 | 274页码:17-25 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) |
| 摘要 | In teleosts, sex is plastic and is influenced by environmental factors. Elevated temperatures have masculinizing effects on the phenotypic sex of certain sensitive species. In this study, we reared genetic XX Japanese flounder at a high temperature (27.5 +/- 0.5 degrees C) and obtained a population of sex-reversal XX males (male ratio, 95.24%). We comparatively analyzed the dynamic characteristics of germ cells and gsdf (gonadal soma-derived factor) expression during sexual differentiation for the experimental (27.5 +/- 0.5 degrees C) and control (18 +/- 0.5 degrees C) groups. The results revealed that the germ cell proliferation inhibited and gsdf expression up-regulated in the experimental group, and the gsdf mRNA and proteins expressed in somatic cells that had direct contact with germline stem cells (with Nanos 2 protein expression) including spermatogonia and oogonia by ISH (in situ hybridization) and IHC (immunohistochemistry). In addition, we also overexpressed the gsdf in XX flounders, and the germ cell number of XX flounders bearing gsdf gene significantly decreased and sometimes disappeared completely, which was consistent with the results from high-temperature induction. Therefore, based on all the results, we speculated that the high expression of gsdf might inhibit germ cell proliferation during sex differentiation, and eventually cause sex reversal in the high-temperature induced masculinization of XX Japanese flounder. |
| 关键词 | High temperature Sex reversal Gsdf Japanese flounder Germline stem cell |
| DOI | 10.1016/j.ygcen.2018.12.012 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] ; Youth Innovation Promotion Association CAS ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; China Postdoctoral Science Foundation[2016M592259] ; China Agriculture Research System[CARS -47] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS -47] ; China Postdoctoral Science Foundation[2016M592259] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] |
| WOS研究方向 | Endocrinology & Metabolism |
| WOS类目 | Endocrinology & Metabolism |
| WOS记录号 | WOS:000460849000003 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/155232 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Shenghang Aquat Product Sci & Technol Co L, Weihai 264200, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nankai Rd, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Yang, Yang,Liu, Qinghua,Xiao, Yongshuang,et al. High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus)[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2019,274:17-25. |
| APA | Yang, Yang.,Liu, Qinghua.,Xiao, Yongshuang.,Xu, Shihong.,Wang, Xueying.,...&Li, Jun.(2019).High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus).GENERAL AND COMPARATIVE ENDOCRINOLOGY,274,17-25. |
| MLA | Yang, Yang,et al."High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus)".GENERAL AND COMPARATIVE ENDOCRINOLOGY 274(2019):17-25. |
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| High temperature inc（14571KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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