Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system | |
Wang Chun1,2 ; Sun Guoxiang1; Li Shuangshuang3; Li Xian1; Liu Ying1
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| 2018-03-01 | |
| 发表期刊 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 2096-5508 |
| 卷号 | 36期号:2页码:414-426 |
| 摘要 | The present study sampled the intestinal content of healthy and unhealthy Atlantic salmon (Salmo salar L.), the ambient water of unhealthy fish, and the biofilter material in the recirculating aquaculture system (RAS) to understand differences in the intestinal microbiota. The V4-V5 regions of the prokaryotic 16S rRNA genes in the samples were analyzed by MiSeq high-throughput sequencing. The fish were adults with no differences in body length or weight. Representative members of the intestinal microbiota were identified. The intestinal microbiota of the healthy fish included Proteobacteria (44.33%), Actinobacteria (17.89%), Bacteroidetes (15.25%), and Firmicutes (9.11%), among which the families Micrococcaceae and Oxalobacteraceae and genera Sphingomonas, Streptomyces, Pedobacter, Janthinobacterium, Burkholderia, and Balneimonas were most abundant. Proteobacteria (70.46%), Bacteroidetes (7.59%), and Firmicutes (7.55%) dominated the microbiota of unhealthy fish, and Chloroflexi (2.71%), and Aliivibrio and Vibrio as well as genera in the family Aeromonadaceae were most strongly represented. Overall, the intestinal hindgut microbiota differed between healthy and unhealthy fish. This study offers a useful tool for monitoring the health status of fish and for screening the utility of probiotics by studying the intestinal microbiota. |
| 关键词 | intestinal microbiota health status Atlantic salmon (Salmo salar L.) recirculating aquaculture system high-throughput pyrosequencing |
| DOI | 10.1007/s00343-017-6203-5 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Project for International S&T Cooperation Program of China[2014DFA31030] ; Earmarked Fund for Modern Agroindustry Technology Research System[CARS-48] ; National Key Technology Research and Development Program of China[2014BAD08B09] ; Qingdao Innovation Talents Program[13-CX-16] ; National Natural Science Foundation of China[31402283] ; National Natural Science Foundation of China[41306152] ; National Natural Science Foundation of China[31472312] ; National Natural Science Foundation of China[31472312] ; National Natural Science Foundation of China[41306152] ; National Natural Science Foundation of China[31402283] ; Qingdao Innovation Talents Program[13-CX-16] ; National Key Technology Research and Development Program of China[2014BAD08B09] ; Earmarked Fund for Modern Agroindustry Technology Research System[CARS-48] ; Project for International S&T Cooperation Program of China[2014DFA31030] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| WOS类目 | Limnology ; Oceanography |
| WOS记录号 | WOS:000431662200021 |
| 出版者 | SCIENCE PRESS |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/154689 |
| 专题 | 海洋生态与环境科学重点实验室 实验海洋生物学重点实验室 海洋生物技术研发中心 |
| 通讯作者 | Liu Ying |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hebei Univ Engn, Coll Energy & Environm Engn, Handan 056038, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang Chun,Sun Guoxiang,Li Shuangshuang,et al. Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(2):414-426. |
| APA | Wang Chun,Sun Guoxiang,Li Shuangshuang,Li Xian,&Liu Ying.(2018).Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(2),414-426. |
| MLA | Wang Chun,et al."Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.2(2018):414-426. |
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| Wang-2017-Intestinal（606KB） | 期刊论文 | 作者接受稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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