The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas

doi:10.3389/fimmu.2018.00284

IOCAS-IR > 实验海洋生物学重点实验室

	The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas
	Liu, Zhaoqun1,2,3 ; Wang, Lingling 1,2; Lv, Zhao 3,4; Zhou, Zhi 5; Wang, Weilin1 ; Li, Meijia3,4 ; Yi, Qilin 1; Qiu, Limei 3; Song, Linsheng 1,2
	2018-02-26
发表期刊	FRONTIERS IN IMMUNOLOGY
卷号	9
文章类型	Article
摘要	It is becoming increasingly clear that neurotransmitters impose direct influence on regulation of the immune process. Recently, a simple but sophisticated neuroendocrine-immune (NEI) system was identified in oyster, which modulated neural immune response via a "nervous-hemocyte"-mediated neuroendocrine immunomodulatory axis (NIA)-like pathway. In the present study, the de novo synthesis of neurotransmitters and their immunomodulation in the hemocytes of oyster Crassostrea gigas were investigated to understand the autocrine/paracrine pathway independent of the nervous system. After hemocytes were exposed to lipopolysaccharide (LPS) stimulation, acetylcholine (ACh), and norepinephrine (NE) in the cell supernatants, both increased to a significantly higher level (2.71-and 2.40-fold, p < 0.05) comparing with that in the control group. The mRNA expression levels and protein activities of choline O-acetyltransferase and dopamine beta-hydroxylase in hemocytes which were involved in the synthesis of ACh and NE were significantly elevated at 1 h after LPS stimulation, while the activities of acetylcholinesterase and monoamine oxidase, two enzymes essential in the metabolic inactivation of ACh and NE, were inhibited. These results demonstrated the existence of the sophisticated intracellular machinery for the generation, release and inactivation of ACh and NE in oyster hemocytes. Moreover, the hemocyte-derived neurotransmitters could in turn regulate the mRNA expressions of tumor necrosis factor (TNF) genes, the activities of superoxide dismutase, catalase and lysosome, and hemocyte phagocytosis. The phagocytic activities of hemocytes, the mRNA expressions of TNF and the activities of key immune-related enzymes were significantly changed after the block of ACh and NE receptors with different kinds of antagonists, suggesting that autocrine/paracrine self-regulation was mediated by transmembrane receptors on hemocyte. The present study proved that oyster hemocyte could de novo synthesize and release cholinergic and adrenergic neurotransmitters, and the hemocyte-derived ACh/NE could then execute a negative regulation on hemocyte phagocytosis and synthesis of immune effectors with similar autocrine/paracrine signaling pathway identified in vertebrate macrophages. Findings in the present study demonstrated that the immune and neuroendocrine system evolved from a common origin and enriched our knowledge on the evolution of NEI system.
关键词	Crassostrea Gigas Autocrine/paracrine Hemocyte Neurotransmitter Membrane Receptor Immune Regulation
DOI	10.3389/fimmu.2018.00284
收录类别	SCI
语种	英语
WOS记录号	WOS:000426069400001
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/154362
专题	实验海洋生物学重点实验室
作者单位	1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Hainan Univ, Minist Educ, Key Lab Trop Biol Resources, Haikou, Hainan, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Zhaoqun,Wang, Lingling,Lv, Zhao,et al. The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2018,9.
APA	Liu, Zhaoqun.,Wang, Lingling.,Lv, Zhao.,Zhou, Zhi.,Wang, Weilin.,...&Song, Linsheng.(2018).The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas.FRONTIERS IN IMMUNOLOGY,9.
MLA	Liu, Zhaoqun,et al."The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas".FRONTIERS IN IMMUNOLOGY 9(2018).

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The cholinergic and （1957KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览