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The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas
Liu, Zhaoqun1,2,3; Wang, Lingling1,2; Lv, Zhao3,4; Zhou, Zhi5; Wang, Weilin1; Li, Meijia3,4; Yi, Qilin1; Qiu, Limei3; Song, Linsheng1,2
2018-02-26
Source PublicationFRONTIERS IN IMMUNOLOGY
Volume9
SubtypeArticle
AbstractIt is becoming increasingly clear that neurotransmitters impose direct influence on regulation of the immune process. Recently, a simple but sophisticated neuroendocrine-immune (NEI) system was identified in oyster, which modulated neural immune response via a "nervous-hemocyte"-mediated neuroendocrine immunomodulatory axis (NIA)-like pathway. In the present study, the de novo synthesis of neurotransmitters and their immunomodulation in the hemocytes of oyster Crassostrea gigas were investigated to understand the autocrine/paracrine pathway independent of the nervous system. After hemocytes were exposed to lipopolysaccharide (LPS) stimulation, acetylcholine (ACh), and norepinephrine (NE) in the cell supernatants, both increased to a significantly higher level (2.71-and 2.40-fold, p < 0.05) comparing with that in the control group. The mRNA expression levels and protein activities of choline O-acetyltransferase and dopamine beta-hydroxylase in hemocytes which were involved in the synthesis of ACh and NE were significantly elevated at 1 h after LPS stimulation, while the activities of acetylcholinesterase and monoamine oxidase, two enzymes essential in the metabolic inactivation of ACh and NE, were inhibited. These results demonstrated the existence of the sophisticated intracellular machinery for the generation, release and inactivation of ACh and NE in oyster hemocytes. Moreover, the hemocyte-derived neurotransmitters could in turn regulate the mRNA expressions of tumor necrosis factor (TNF) genes, the activities of superoxide dismutase, catalase and lysosome, and hemocyte phagocytosis. The phagocytic activities of hemocytes, the mRNA expressions of TNF and the activities of key immune-related enzymes were significantly changed after the block of ACh and NE receptors with different kinds of antagonists, suggesting that autocrine/paracrine self-regulation was mediated by transmembrane receptors on hemocyte. The present study proved that oyster hemocyte could de novo synthesize and release cholinergic and adrenergic neurotransmitters, and the hemocyte-derived ACh/NE could then execute a negative regulation on hemocyte phagocytosis and synthesis of immune effectors with similar autocrine/paracrine signaling pathway identified in vertebrate macrophages. Findings in the present study demonstrated that the immune and neuroendocrine system evolved from a common origin and enriched our knowledge on the evolution of NEI system.
KeywordCrassostrea Gigas Autocrine/paracrine Hemocyte Neurotransmitter Membrane Receptor Immune Regulation
DOI10.3389/fimmu.2018.00284
Indexed BySCI
Language英语
WOS IDWOS:000426069400001
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/154362
Collection实验海洋生物学重点实验室
Affiliation1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Hainan Univ, Minist Educ, Key Lab Trop Biol Resources, Haikou, Hainan, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Zhaoqun,Wang, Lingling,Lv, Zhao,et al. The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2018,9.
APA Liu, Zhaoqun.,Wang, Lingling.,Lv, Zhao.,Zhou, Zhi.,Wang, Weilin.,...&Song, Linsheng.(2018).The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas.FRONTIERS IN IMMUNOLOGY,9.
MLA Liu, Zhaoqun,et al."The cholinergic and adrenergic autocrine signaling Pathway Mediates immunomodulation in Oyster Crassostrea gigas".FRONTIERS IN IMMUNOLOGY 9(2018).
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