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Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J
Sun, Yuhao1,2,3; Chen, Xiaolin1,3; Cheng, Ziqiang4; Liu, Song1,3; Yu, Huahua1,3; Wang, Xueqin1,3; Li, Pengcheng1,3
2017-11-01
Source PublicationMARINE DRUGS
Volume15Issue:11
SubtypeArticle
AbstractIn this study, polysaccharides from Grateloupia filicinia (GFP) were extracted and several low molecular weight (Mw) G. filicina polysaccharides (LGFPs) were prepared by the hydrogen peroxide (H2O2) oxidation method. Additionally, the effect of different experimental conditions on the degradation of GFP was determined. Results showed that the GFP degradation rate was positively related to H2O2 concentration and temperature, and negatively related to pH. Chemical analysis and Fourier transform infrared spectra (FT-IR) of GFP and LGFPs showed that the degradation caused a slight decrease of total sugar and sulfate content. However, there was no obvious change for monosaccharide contents. Then, the anti-ALV-J activity of GFP and LGFPs were determined in vitro. Results revealed that all of the samples could significantly inhibit ALV-J and lower Mw LGFPs exhibited a stronger suppression, and that the fraction LGFP-3 with Mw 8.7 kDa had the best effect. In addition, the reaction phase assays showed that the inhibition effect was mainly because of the blocking virus adsorption to host cells. Moreover, real-time PCR, western-blot, and IFA were further applied to evaluate the blocking effects of LGFP-3. Results showed that the gene relative expression and gp85 protein for LGFPS-3 groups were all reduced. Data from IFA showed that there was less virus infected cells for 1000 and 200 g/mL LGFPS-3 groups when compared to virus control. Therefore, lower Mw polysaccharides from G. filicina might supply a good choice for ALV-J prevention and treatment.
KeywordGrateloupia Filicina Degradation Low Molecular Weight Polysaccharides Antiviral Activity Alv-j
DOI10.3390/md15110345
Indexed BySCI
Language英语
WOS IDWOS:000416600200016
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143282
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
海洋环境工程技术研究发展中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Shandong Agr Univ, Coll Anim Sci & Vet Med, 61 Daizong Rd, Tai An 271018, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Yuhao,Chen, Xiaolin,Cheng, Ziqiang,et al. Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J[J]. MARINE DRUGS,2017,15(11).
APA Sun, Yuhao.,Chen, Xiaolin.,Cheng, Ziqiang.,Liu, Song.,Yu, Huahua.,...&Li, Pengcheng.(2017).Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J.MARINE DRUGS,15(11).
MLA Sun, Yuhao,et al."Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J".MARINE DRUGS 15.11(2017).
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