IOCAS-IR  > 海洋生物技术研发中心
Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J
Sun, Yuhao1,2,3; Chen, Xiaolin1,3; Cheng, Ziqiang4; Liu, Song1,3; Yu, Huahua1,3; Wang, Xueqin1,3; Li, Pengcheng1,3
2017-11-01
发表期刊MARINE DRUGS
卷号15期号:11
文章类型Article
摘要In this study, polysaccharides from Grateloupia filicinia (GFP) were extracted and several low molecular weight (Mw) G. filicina polysaccharides (LGFPs) were prepared by the hydrogen peroxide (H2O2) oxidation method. Additionally, the effect of different experimental conditions on the degradation of GFP was determined. Results showed that the GFP degradation rate was positively related to H2O2 concentration and temperature, and negatively related to pH. Chemical analysis and Fourier transform infrared spectra (FT-IR) of GFP and LGFPs showed that the degradation caused a slight decrease of total sugar and sulfate content. However, there was no obvious change for monosaccharide contents. Then, the anti-ALV-J activity of GFP and LGFPs were determined in vitro. Results revealed that all of the samples could significantly inhibit ALV-J and lower Mw LGFPs exhibited a stronger suppression, and that the fraction LGFP-3 with Mw 8.7 kDa had the best effect. In addition, the reaction phase assays showed that the inhibition effect was mainly because of the blocking virus adsorption to host cells. Moreover, real-time PCR, western-blot, and IFA were further applied to evaluate the blocking effects of LGFP-3. Results showed that the gene relative expression and gp85 protein for LGFPS-3 groups were all reduced. Data from IFA showed that there was less virus infected cells for 1000 and 200 g/mL LGFPS-3 groups when compared to virus control. Therefore, lower Mw polysaccharides from G. filicina might supply a good choice for ALV-J prevention and treatment.
关键词Grateloupia Filicina Degradation Low Molecular Weight Polysaccharides Antiviral Activity Alv-j
DOI10.3390/md15110345
收录类别SCI
语种英语
WOS记录号WOS:000416600200016
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143282
专题海洋生物技术研发中心
实验海洋生物学重点实验室
海洋环境工程技术研究发展中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Shandong Agr Univ, Coll Anim Sci & Vet Med, 61 Daizong Rd, Tai An 271018, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Sun, Yuhao,Chen, Xiaolin,Cheng, Ziqiang,et al. Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J[J]. MARINE DRUGS,2017,15(11).
APA Sun, Yuhao.,Chen, Xiaolin.,Cheng, Ziqiang.,Liu, Song.,Yu, Huahua.,...&Li, Pengcheng.(2017).Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J.MARINE DRUGS,15(11).
MLA Sun, Yuhao,et al."Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J".MARINE DRUGS 15.11(2017).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Degradation of Polys(5976KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sun, Yuhao]的文章
[Chen, Xiaolin]的文章
[Cheng, Ziqiang]的文章
百度学术
百度学术中相似的文章
[Sun, Yuhao]的文章
[Chen, Xiaolin]的文章
[Cheng, Ziqiang]的文章
必应学术
必应学术中相似的文章
[Sun, Yuhao]的文章
[Chen, Xiaolin]的文章
[Cheng, Ziqiang]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。