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Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)
Li, Busu1,2,3,4; Meng, Jie1,2,5; Li, Li1,2,5; Liu, Sheng1,2,3,4; Wang, Ting1,2,3,4; Zhang, Guofan1,2,4
2017-09-06
发表期刊JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷号65期号:35页码:7764-7773
文章类型Article
摘要High glycogen levels in the Pacific oyster (Crassostrea gigas) contribute to its flavor, quality, and hardiness. Glycogenin (CgGN) is the priming glucosyltransferase that initiates glycogen biosynthesis. We characterized the full sequence and function of C. gigas CgGN. Three CgGN isoforms (CgGN-alpha, beta, and gamma) containing alternative exon regions were isolated. CgGN expression varied seasonally in the adductor muscle and gonadal area and was the highest in the adductor muscle. Autoglycosylation of CgGN can interact with glycogen synthase (CgGS) to complete glycogen synthesis. Subcellular localization analysis showed that CgGN isoforms and CgGS were located in the cytoplasm. Additionally, a site-directed mutagenesis experiment revealed that the Tyr200Phe and Tyr202Phe mutations could affect CgGN autoglycosylation. This is the first study of glycogenin function in marine bivalves. These findings will improve our understanding of glycogen synthesis and accumulation mechanisms in mollusks. The data are potentially useful for breeding high-glycogen oysters.
关键词Crassostrea Gigas Glycogenin Glycogen Biosynthesis Glycogen Synthase Alternative Splicing
DOI10.1021/acs.jafc.7b02720
收录类别SCI
语种英语
WOS记录号WOS:000410255400020
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143165
专题海洋生物技术研发中心
实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266000, Shandong, Peoples R China
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Li, Busu,Meng, Jie,Li, Li,et al. Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(35):7764-7773.
APA Li, Busu,Meng, Jie,Li, Li,Liu, Sheng,Wang, Ting,&Zhang, Guofan.(2017).Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas).JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(35),7764-7773.
MLA Li, Busu,et al."Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.35(2017):7764-7773.
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