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Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)
Li, Busu1,2,3,4; Meng, Jie1,2,5; Li, Li1,2,5; Liu, Sheng1,2,3,4; Wang, Ting1,2,3,4; Zhang, Guofan1,2,4
2017-09-06
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume65Issue:35Pages:7764-7773
SubtypeArticle
AbstractHigh glycogen levels in the Pacific oyster (Crassostrea gigas) contribute to its flavor, quality, and hardiness. Glycogenin (CgGN) is the priming glucosyltransferase that initiates glycogen biosynthesis. We characterized the full sequence and function of C. gigas CgGN. Three CgGN isoforms (CgGN-alpha, beta, and gamma) containing alternative exon regions were isolated. CgGN expression varied seasonally in the adductor muscle and gonadal area and was the highest in the adductor muscle. Autoglycosylation of CgGN can interact with glycogen synthase (CgGS) to complete glycogen synthesis. Subcellular localization analysis showed that CgGN isoforms and CgGS were located in the cytoplasm. Additionally, a site-directed mutagenesis experiment revealed that the Tyr200Phe and Tyr202Phe mutations could affect CgGN autoglycosylation. This is the first study of glycogenin function in marine bivalves. These findings will improve our understanding of glycogen synthesis and accumulation mechanisms in mollusks. The data are potentially useful for breeding high-glycogen oysters.
KeywordCrassostrea Gigas Glycogenin Glycogen Biosynthesis Glycogen Synthase Alternative Splicing
DOI10.1021/acs.jafc.7b02720
Indexed BySCI
Language英语
WOS IDWOS:000410255400020
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143165
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266000, Shandong, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences;  Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Busu,Meng, Jie,Li, Li,et al. Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(35):7764-7773.
APA Li, Busu,Meng, Jie,Li, Li,Liu, Sheng,Wang, Ting,&Zhang, Guofan.(2017).Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas).JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(35),7764-7773.
MLA Li, Busu,et al."Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.35(2017):7764-7773.
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