IOCAS-IR  > 海洋生态与环境科学重点实验室
Identification and expression characterization of WntA during intestinal regeneration in the sea cucumber Apostichopus japonicus
Li, Xiaoni1,2,3; Sun, Lina1,3; Yang, Hongsheng1,3; Zhang, Libin1,3; Miao, Ting1,3; Xing, Lili1,2,3; Huo, Da1,2,3
2017-08-01
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷号210页码:55-63
文章类型Article
摘要Wnt genes encode secreted glycoproteins that act as signaling molecules; these molecules direct cell proliferation, migration, differentiation and survival during animal development, maintenance of homeostasis and regeneration. At present, although the regeneration mechanism in Apostichopus japonicus has been studied, there is a little research on the Wnt signaling pathway in A. japonicus. To understand the potential role of the Wnt signaling pathway in A. japonicus, we cloned and sequenced the WntA gene in A. japonicus. Protein localization analysis showed that WntA protein was ubiquitously expressed in epidermal cells, the muscle and submucosa of the intestinal tissue. After stimulation and evisceration, the dynamic changes in expression of the WntA gene and protein showed that WntA was constitutively expressed during different stages of intestine regeneration in A. japonicus, with higher levels during the early wound healing stage and late lumen formation in the residual and nascent intestinal tissues, indicating its response to intestinal regeneration. Simultaneously, cell proliferation and apoptosis analysis showed that the patterns of cell proliferation were similar to the patterns of WntA protein expression during different intestinal regeneration stages in this organism. Taken together, these results suggested that WntA might participate in intestinal regeneration and may be connected with cell proliferation, apoptosis in different intestinal layers. This research could establish a basis for further examination of WntA functions in A. japonicus and Wnt genes in other echinoderms.
关键词Echinoderm Regeneration Evisceration Proliferation Apoptosis
DOI10.1016/j.cbpb.2017.06.005
收录类别SCI
语种英语
WOS记录号WOS:000406565500007
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143093
专题海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Li, Xiaoni,Sun, Lina,Yang, Hongsheng,et al. Identification and expression characterization of WntA during intestinal regeneration in the sea cucumber Apostichopus japonicus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2017,210:55-63.
APA Li, Xiaoni.,Sun, Lina.,Yang, Hongsheng.,Zhang, Libin.,Miao, Ting.,...&Huo, Da.(2017).Identification and expression characterization of WntA during intestinal regeneration in the sea cucumber Apostichopus japonicus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,210,55-63.
MLA Li, Xiaoni,et al."Identification and expression characterization of WntA during intestinal regeneration in the sea cucumber Apostichopus japonicus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 210(2017):55-63.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Identification and e(2782KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Xiaoni]的文章
[Sun, Lina]的文章
[Yang, Hongsheng]的文章
百度学术
百度学术中相似的文章
[Li, Xiaoni]的文章
[Sun, Lina]的文章
[Yang, Hongsheng]的文章
必应学术
必应学术中相似的文章
[Li, Xiaoni]的文章
[Sun, Lina]的文章
[Yang, Hongsheng]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Identification and expression characterization of WntA during intestinal regeneration in the sea cucumber Apostichopus japonicus.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。