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| Molecular characterization and transcriptional analysis of a crustacean heat shock protein 10 gene in shrimp Litopenaeus vannamei | |
Wang, M-Q1; Wang, B-J1; Jiang, Ke-Y1; Liu, M.1; Han, Si-Y1,3; Wang, L.1,2
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| 2017 | |
| 发表期刊 | ISJ-INVERTEBRATE SURVIVAL JOURNAL
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| 卷号 | 14页码:404-413 |
| 文章类型 | Article |
| 摘要 | Heat shock proteins (HSPs) are the most abundant and ubiquitous soluble intracellular proteins which conserved phylogenetically in all living organisms from archaebacteria to humans. Recent research achievements indicated that HSP10s might not only be involved in the responses to environmental stresses, but also play a pivotal role in the host defenses mechanism. In the present study, a cDNA of 715 bp for the Pacific white shrimp Litopenaeus vannamei HSP10 (designated as LvHSP10) was cloned via rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of LvHSP10 contained an open reading frame (ORF) of 309 bp, which encoded a protein of 102 amino acids. The protein sequence of LvHSP10 shared over 80 % similarity with previously identified HSP10s. There were a CPN10 domain and a chaperonins HSP10/CPN10 signature in the protein sequence of LvHSP10. The mRNA transcripts of LvHSP10 were constitutively expressed in all the tested tissues, including eyestalk, gill, gonad, heart, hemocytes, hepatopancreas, intestine, muscle, nerve and stomach, with the highest expression level in hepatopancreas. The mRNA expression profiles of LvHSP10 in hepatopancreas could be significantly induced by the stimulation of Vibrio parahaemolyticus, white spot syndrome virus (WSSV), and low and high pH challenge. These results provided useful information of the potential roles of LvHSP10 in the defense mechanism of shrimp against various biological stimulations and multiple environmental stresses. |
| 关键词 | Heat Shock Protein 10 Hepatopancreas Litopenaeus Vannamei |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000414538400001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143060 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, M-Q,Wang, B-J,Jiang, Ke-Y,et al. Molecular characterization and transcriptional analysis of a crustacean heat shock protein 10 gene in shrimp Litopenaeus vannamei[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2017,14:404-413. |
| APA | Wang, M-Q,Wang, B-J,Jiang, Ke-Y,Liu, M.,Han, Si-Y,&Wang, L..(2017).Molecular characterization and transcriptional analysis of a crustacean heat shock protein 10 gene in shrimp Litopenaeus vannamei.ISJ-INVERTEBRATE SURVIVAL JOURNAL,14,404-413. |
| MLA | Wang, M-Q,et al."Molecular characterization and transcriptional analysis of a crustacean heat shock protein 10 gene in shrimp Litopenaeus vannamei".ISJ-INVERTEBRATE SURVIVAL JOURNAL 14(2017):404-413. |
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| Molecular characteri（1177KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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