IOCAS-IR  > 海洋腐蚀与防护研究发展中心
Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles
Zeng, Yan1; Zhang, Dun1; Qi, Peng1; Zheng, Laibao1,2
2017-12-01
Source PublicationMICROCHIMICA ACTA
Volume184Issue:12Pages:4809-4815
SubtypeArticle
AbstractThe authors describe a strategy for colorimetric detection of DNA. It is making use of a target catalyzed DNA nanostructure assembly and gold nanoparticles. The assay comprises the following steps: (a) A programmed DNA nanostructure is assembled from three auxiliary hairpin structure DNAs and the single stranded DNA (ssDNA; the target/analyte); (b) in the presence of target DNA, these three hairpin DNAs are opened, thereby activating a catalytic self-assembly process via a target assisted toe-hold strand displacement reaction; (c) The formed DNA nanostructures are mixed with gold nanoparticles. As the DNA nanostructure is less stabilized without ssDNA sticky ends, it cannot prevent the gold nanoparticles (AuNPs) to undergo salt-induced aggregation which is accompanied by a color change from purple to blue; (d) The color change of the colloid solution can be read out with bare eyes or instrumentally. The detection limit by using UV-vis spectrometry is 0.6 pM of target DNA. This is comparable to previously AuNP-based methods. Thus, this assay provides free modification detection based on DNA nanostructure without sophisticated procedures. Conceivably, the method can be applied to numerous other DNA targets.
KeywordDnadetection Goldnanoparticle Colorimetry Self-assembly Dna Nanostructure
DOI10.1007/s00604-017-2463-1
Indexed BySCI
Language英语
WOS IDWOS:000414547400029
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143036
Collection海洋腐蚀与防护研究发展中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Yan,Zhang, Dun,Qi, Peng,et al. Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles[J]. MICROCHIMICA ACTA,2017,184(12):4809-4815.
APA Zeng, Yan,Zhang, Dun,Qi, Peng,&Zheng, Laibao.(2017).Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles.MICROCHIMICA ACTA,184(12),4809-4815.
MLA Zeng, Yan,et al."Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles".MICROCHIMICA ACTA 184.12(2017):4809-4815.
Files in This Item:
File Name/Size DocType Version Access License
Colorimetric detecti(1538KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zeng, Yan]'s Articles
[Zhang, Dun]'s Articles
[Qi, Peng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zeng, Yan]'s Articles
[Zhang, Dun]'s Articles
[Qi, Peng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zeng, Yan]'s Articles
[Zhang, Dun]'s Articles
[Qi, Peng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.