| Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway | |
Tang, Xueying1,2,3,5; Huang, Baoyu1,3,5 ; Zhang, Linlin1,3,5; Li, Li1,4,5; Zhang, Guofan1,3,5
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| 2017-07-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 卷号 | 72页码:21-29 |
| 文章类型 | Article |
| 摘要 | Interleukin-1 receptor-associated kinases (IRAKs) play important roles in MyD88-dependent TLR signaling, the crucial innate immune pathway in molluscs. In this study, we examined the full-length IRAK4 genetic sequence in the Pacific oyster (Crassostrea gigas) by molecular cloning. Phylogenetic analysis revealed that CgIRAK4 is most closely related to Mytilus edulis, and forms a Glade with other molluscs. CgIRAK4 transcripts are widely expressed in all tissues, with the highest expression observed in the hemocytes and gill. Moreover, CgIRAK4 is significantly upregulated after Oyster herpesvirus-1 microvariant (0sHV-1 mu var), Vibrio alginolyticus, and poly I:C challenge. Yeast two-hybrid and co-immunoprecipitation assays reveal that the CgIRAK4 death domain is necessary to mediate interaction between CgIRAK4 and two CgMyD88 isoforms. In addition, CgIRAK4 overexpression cannot induce NF-kappa B transcriptional activity, but blocks that induced by CgMyD88 in HEK293T cells. These findings elucidate the mechanisms of MyD88-dependent TLR signaling in molluscs, and the differences in IRAK-mediated pathway activation between invertebrates and vertebrates. (C) 2017 Elsevier Ltd. All rights reserved. |
| 关键词 | Crassostrea Gigas Irak4 Myd88 Tlr Nf-kappa b |
| DOI | 10.1016/j.dci.2017.02.004 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000400038100003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/137057 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China 5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Tang, Xueying,Huang, Baoyu,Zhang, Linlin,et al. Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,72:21-29. |
| APA | Tang, Xueying,Huang, Baoyu,Zhang, Linlin,Li, Li,&Zhang, Guofan.(2017).Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,72,21-29. |
| MLA | Tang, Xueying,et al."Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 72(2017):21-29. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Molecular characteri（2199KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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