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Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress
Liu, Zhaoqun1,2,3; Wang, Lingling4; Zhou, Zhi5; Liu, Yu4; Dong, Miren4; Wang, Weilin1,2,3; Song, Xiaorui4; Wang, Mengqiang1; Gao, Qiang1,4; Song, Linsheng2,4
2017-08-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume73Pages:52-60
SubtypeArticle
AbstractBacterial infection and heat stress, as two major environmental threats of marine molluscs, could affect larval development and dramatically promote mortality of oysters. In the present study, next-generation sequencing, together with determinations of mRNA expression and measurements of enzyme activities, were employed to understand the response patterns of oyster larvae under acute heat and bacterial stress. After RNA-seq, a total of 9472 differentially expressed genes including 4895 significantly up regulated ones and 4577 significantly down-regulated ones were obtained from 12 transcriptome libraries. GO overrepresentation analysis of the up-regulated genes revealed that the neuroendocrine immunomodulation pathway was activated after acute heat and bacterial stimulation, in which the catecholaminergic regulation played an important role. GO overrepresentation analysis of the down regulated genes suggested that the immune capacity of Crassostrea gigas larvae was suppressed under stress, which was further validated since superoxide dismutase (SOD) and phenoloxidase (PO) activities in the total protein extract of larvae decreased dramatically after stress. Moreover, the shell formation of trochophore was inhibited and severe mortality was caused after acute heat and bacterial stress. These results collectively indicated that acute heat and bacterial stress could significantly inhibit larval development and suppress immune response of oyster C. gigas larvae. And the neuroendocrine immunomodulation, especially the catecholaminergic regulation, played an indispensable role in the stress response of molluscan larvae. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Larvae Transcriptomic Analysis Acute Heat And Bacterial Stress Catecholaminergic System Immunomodulation
DOI10.1016/j.dci.2017.03.005
Indexed BySCI
Language英语
WOS IDWOS:000401380400007
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136861
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266200, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
5.Hainan Univ, Minist Educ, Key Lab Trop Biol Resources, Haikou 570228, Peoples R China
First Author Affilication中国科学院海洋研究所
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Liu, Zhaoqun,Wang, Lingling,Zhou, Zhi,et al. Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,73:52-60.
APA Liu, Zhaoqun.,Wang, Lingling.,Zhou, Zhi.,Liu, Yu.,Dong, Miren.,...&Song, Linsheng.(2017).Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,73,52-60.
MLA Liu, Zhaoqun,et al."Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 73(2017):52-60.
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