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A Carbonic Anhydrase Serves as an Important Acid-Base Regulator in Pacific Oyster Crassostrea gigas Exposed to Elevated CO2: Implication for Physiological Responses of Mollusk to Ocean Acidification
Wang, Xiudan1,2; Wang, Mengqiang1; Jia, Zhihao1,2; Qiu, Limei1; Wang, Lingling3; Zhang, Anguo3; Song, Linsheng3
2017-02-01
Source PublicationMARINE BIOTECHNOLOGY
Volume19Issue:1Pages:22-35
SubtypeArticle
AbstractCarbonic anhydrases (CAs) have been demonstrated to play an important role in acid-base regulation in vertebrates. However, the classification and modulatory function of CAs in marine invertebrates, especially their responses to ocean acidification remain largely unknown. Here, a cytosolic alpha-CA (designated as CgCAII-1) was characterized from Pacific oyster Crassostrea gigas and its molecular activities against CO2 exposure were investigated. CgCAII-1 possessed a conserved CA catalytic domain, with high similarity to invertebrate cytoplasmic or mitochondrial alpha-CAs. Recombinant CgCAII-1 could convert CO2 to HCO3 (-) with calculated activity as 0.54 x 10(3) U/mg, which could be inhibited by acetazolamide (AZ). The mRNA transcripts of CgCAII-1 in muscle, mantle, hepatopancreas, gill, and hemocytes increased significantly after exposure to elevated CO2. CgCAII-1 could interact with the hemocyte membrane proteins and the distribution of CgCAII-1 protein became more concentrated and dense in gill and mantle under CO2 exposure. The intracellular pH (pHi) of hemocytes under CO2 exposure increased significantly (p < 0.05) and CA inhibition reduced the pHi value. Besides, there was no increase in CA activity in gill and mantle after CO2 exposure. The impact of CO2 exposure on CA activity coupled with the mRNA expression level and protein translocation of CgCAII-1 provided evidences that CgCAII-1 could respond to ocean acidification and participate in acid-base regulation. Such cytoplasmic CA-based physiological regulation mechanism might explain other physiological responses of marine organisms to OA.
KeywordCarbonic Anhydrase Oyster Acid-base Regulation Co2 Exposure Ocean Acidification
DOI10.1007/s10126-017-9734-z
Indexed BySCI
Language英语
WOS IDWOS:000395086900003
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136781
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xiudan,Wang, Mengqiang,Jia, Zhihao,et al. A Carbonic Anhydrase Serves as an Important Acid-Base Regulator in Pacific Oyster Crassostrea gigas Exposed to Elevated CO2: Implication for Physiological Responses of Mollusk to Ocean Acidification[J]. MARINE BIOTECHNOLOGY,2017,19(1):22-35.
APA Wang, Xiudan.,Wang, Mengqiang.,Jia, Zhihao.,Qiu, Limei.,Wang, Lingling.,...&Song, Linsheng.(2017).A Carbonic Anhydrase Serves as an Important Acid-Base Regulator in Pacific Oyster Crassostrea gigas Exposed to Elevated CO2: Implication for Physiological Responses of Mollusk to Ocean Acidification.MARINE BIOTECHNOLOGY,19(1),22-35.
MLA Wang, Xiudan,et al."A Carbonic Anhydrase Serves as an Important Acid-Base Regulator in Pacific Oyster Crassostrea gigas Exposed to Elevated CO2: Implication for Physiological Responses of Mollusk to Ocean Acidification".MARINE BIOTECHNOLOGY 19.1(2017):22-35.
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