IOCAS-IR  > 海洋生态与环境科学重点实验室
Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)
Zhang, Qianqian1; Agatha, Sabine2; Zhang, Wuchang3; Dong, Jun1; Yu, Ying3; Jiao, Nianzhi4; Gong, Jun1
AbstractTo improve understanding of diversity, phylogeny and evolution in tintinnid ciliates, it is essential to link multiple molecular markers with properly identified and documented morphospecies. Accordingly, 54 tintinnid morphospecies/isolates mainly from the Yellow and East China Seas were collected and analysed. Using single-cell approaches, sequences were obtained for three rDNA loci (18S, ITS1-5.8S-ITS2, D1-D5 region of 28S). Twenty-six tintinnid morphospecies (29 isolates) are documented by micrographs, measurements, morphologically described, and compared with the original species description. Three rDNA loci-based phylogenetic analyses were then performed for these identified isolates. Sequences from 25 unidentified species/isolates were also included in the comparison of the three rDNA loci. Ribosomal DNA genes of the genus Leprotintinnus were analysed for the first time, showing that Leprotintinnus was closely related to Tintinnopsis radix and branched distinctly apart from the family Tintinnidiidae. Four novel clades (VI to IX) of the Tintinnopsis complex emerged in the 18S genealogies. Analyses of the relative variability in the ITS and 28S regions vs. the 18S rDNA showed that the ITS1-5.8S-ITS2 and ITS2 regions well co-varied with the 18S rDNA when the variations of the latter were less than 3%, whereas at difference of less than 1%, no correlation was found between the compared loci. These findings highlight the difficulties in using variable locus-based cut-off divergences in circumscribing tintinnid morphospecies.
KeywordBarcoding Genetic Diversity Marine Protists Microzooplankton Multiple-locus Phylogeny
Indexed BySCI
WOS IDWOS:000395079400009
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Salzburg Univ, Dept Ecol & Evolut, A-5020 Salzburg, Austria
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
4.Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qianqian,Agatha, Sabine,Zhang, Wuchang,et al. Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2017,64(2):226-241.
APA Zhang, Qianqian.,Agatha, Sabine.,Zhang, Wuchang.,Dong, Jun.,Yu, Ying.,...&Gong, Jun.(2017).Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida).JOURNAL OF EUKARYOTIC MICROBIOLOGY,64(2),226-241.
MLA Zhang, Qianqian,et al."Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)".JOURNAL OF EUKARYOTIC MICROBIOLOGY 64.2(2017):226-241.
Files in This Item:
File Name/Size DocType Version Access License
Three rDNA Loci-Base(1797KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Qianqian]'s Articles
[Agatha, Sabine]'s Articles
[Zhang, Wuchang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Qianqian]'s Articles
[Agatha, Sabine]'s Articles
[Zhang, Wuchang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Qianqian]'s Articles
[Agatha, Sabine]'s Articles
[Zhang, Wuchang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates.pdf
Format: Adobe PDF
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.