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Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)
Zhang, Qianqian1; Agatha, Sabine2; Zhang, Wuchang3; Dong, Jun1; Yu, Ying3; Jiao, Nianzhi4; Gong, Jun1
2017-03-01
发表期刊JOURNAL OF EUKARYOTIC MICROBIOLOGY
卷号64期号:2页码:226-241
文章类型Article
摘要To improve understanding of diversity, phylogeny and evolution in tintinnid ciliates, it is essential to link multiple molecular markers with properly identified and documented morphospecies. Accordingly, 54 tintinnid morphospecies/isolates mainly from the Yellow and East China Seas were collected and analysed. Using single-cell approaches, sequences were obtained for three rDNA loci (18S, ITS1-5.8S-ITS2, D1-D5 region of 28S). Twenty-six tintinnid morphospecies (29 isolates) are documented by micrographs, measurements, morphologically described, and compared with the original species description. Three rDNA loci-based phylogenetic analyses were then performed for these identified isolates. Sequences from 25 unidentified species/isolates were also included in the comparison of the three rDNA loci. Ribosomal DNA genes of the genus Leprotintinnus were analysed for the first time, showing that Leprotintinnus was closely related to Tintinnopsis radix and branched distinctly apart from the family Tintinnidiidae. Four novel clades (VI to IX) of the Tintinnopsis complex emerged in the 18S genealogies. Analyses of the relative variability in the ITS and 28S regions vs. the 18S rDNA showed that the ITS1-5.8S-ITS2 and ITS2 regions well co-varied with the 18S rDNA when the variations of the latter were less than 3%, whereas at difference of less than 1%, no correlation was found between the compared loci. These findings highlight the difficulties in using variable locus-based cut-off divergences in circumscribing tintinnid morphospecies.
关键词Barcoding Genetic Diversity Marine Protists Microzooplankton Multiple-locus Phylogeny
DOI10.1111/jeu.12354
收录类别SCI
语种英语
WOS记录号WOS:000395079400009
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136763
专题海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Salzburg Univ, Dept Ecol & Evolut, A-5020 Salzburg, Austria
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
4.Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China
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Zhang, Qianqian,Agatha, Sabine,Zhang, Wuchang,et al. Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2017,64(2):226-241.
APA Zhang, Qianqian.,Agatha, Sabine.,Zhang, Wuchang.,Dong, Jun.,Yu, Ying.,...&Gong, Jun.(2017).Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida).JOURNAL OF EUKARYOTIC MICROBIOLOGY,64(2),226-241.
MLA Zhang, Qianqian,et al."Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)".JOURNAL OF EUKARYOTIC MICROBIOLOGY 64.2(2017):226-241.
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