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Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei
Wang, Lin1; Chen, Hao1,3; Xu, Jianchao1,3; Xu, Qingsong2; Wang, Mengqiang1; Zhao, Depeng2,4; Wang, Lingling2; Song, Linsheng2
2017-03-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号62页码:164-174
文章类型Article
摘要A robust immune response against invading pathogens is crucial for host to survive, which depends greatly on the well balance of metabolism. Increasing evidence has "indicated that some metabolic hormones, such as insulin, could modulate immune responses directly. Crustacean hyperglycemic hormone (CHH) family is a group of ecdysozoans-specific peptide hormone involved in glucose metabolism and other biological events. In the present study, two members of CHH family (designated as LvCHH I and LvCHH II) in shrimp Litopenaeus vannamei with one and two crustacean neurohormone domains respectively were chosen to investigate their putative modulatory roles in both glucose metabolism and immune response. LvCHH I and LvCHH II were both expressed in the sinus gland and lamina ganglionalis of eyestalks and were significantly induced after white spot syndrome virus (WSSV) infection. Meanwhile, significant increases of hemolymph glucose levels were observed in shrimp at 12 and 24 h after WSSV infection while the glucose inside the hemocytes decreased at 6 h and then increased at 12 h. Gain-of-function of rLvCHHs was subsequently conducted in vivo by injecting the recombinant proteins (rLvCHH I and rLvCHH II). The hemolymph glucose increased significantly from 0.5 h to 3 h after the shrimps received an injection of rLvCHH I, while it decreased at 0.5 h and increased afterward at 3 h post rLvCHH II injection. At the meantime, significant decreases of reactive oxygen species level in hemocytes were observed at 3 h and 6 h post rLvCHH I injection, while it remained unchanged in rLvCHH II injection group. rLvCHH I and rLvCHH II could bind to the cytomembrane of primary shrimp hemocytes in vitro, and the expressions of superoxide dismutase and LvRelish increased when the hemocytes were incubated with rLvCHH I for 3 h. Meanwhile, the expression of antimicrobial peptides, crustin and penaeidin4, were also induced by rLvCHH I and rLvCHH II. These results demonstrated that host immune response, in addition to glucose metabolism, could be directly modulated by LvCHH family, and the present study provided new insights into the immunomodulation role of metabolic hormones in invertebrate. (C) 2017 Elsevier Ltd. All rights reserved.
关键词Shrimp Wssv Infection Crustacean Hyperglycemic Hormones Glucose Metabolism Immunomodulation
DOI10.1016/j.fsi.2017.01.007
收录类别SCI
语种英语
WOS记录号WOS:000395955800018
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136760
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Experimental Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Cotrol, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Poltech Univ, Dalian 116034, Peoples R China
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Wang, Lin,Chen, Hao,Xu, Jianchao,et al. Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2017,62:164-174.
APA Wang, Lin.,Chen, Hao.,Xu, Jianchao.,Xu, Qingsong.,Wang, Mengqiang.,...&Song, Linsheng.(2017).Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,62,164-174.
MLA Wang, Lin,et al."Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 62(2017):164-174.
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