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Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei
Wang, Lin1; Chen, Hao1,3; Xu, Jianchao1,3; Xu, Qingsong2; Wang, Mengqiang1; Zhao, Depeng2,4; Wang, Lingling2; Song, Linsheng2
2017-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume62Pages:164-174
SubtypeArticle
AbstractA robust immune response against invading pathogens is crucial for host to survive, which depends greatly on the well balance of metabolism. Increasing evidence has "indicated that some metabolic hormones, such as insulin, could modulate immune responses directly. Crustacean hyperglycemic hormone (CHH) family is a group of ecdysozoans-specific peptide hormone involved in glucose metabolism and other biological events. In the present study, two members of CHH family (designated as LvCHH I and LvCHH II) in shrimp Litopenaeus vannamei with one and two crustacean neurohormone domains respectively were chosen to investigate their putative modulatory roles in both glucose metabolism and immune response. LvCHH I and LvCHH II were both expressed in the sinus gland and lamina ganglionalis of eyestalks and were significantly induced after white spot syndrome virus (WSSV) infection. Meanwhile, significant increases of hemolymph glucose levels were observed in shrimp at 12 and 24 h after WSSV infection while the glucose inside the hemocytes decreased at 6 h and then increased at 12 h. Gain-of-function of rLvCHHs was subsequently conducted in vivo by injecting the recombinant proteins (rLvCHH I and rLvCHH II). The hemolymph glucose increased significantly from 0.5 h to 3 h after the shrimps received an injection of rLvCHH I, while it decreased at 0.5 h and increased afterward at 3 h post rLvCHH II injection. At the meantime, significant decreases of reactive oxygen species level in hemocytes were observed at 3 h and 6 h post rLvCHH I injection, while it remained unchanged in rLvCHH II injection group. rLvCHH I and rLvCHH II could bind to the cytomembrane of primary shrimp hemocytes in vitro, and the expressions of superoxide dismutase and LvRelish increased when the hemocytes were incubated with rLvCHH I for 3 h. Meanwhile, the expression of antimicrobial peptides, crustin and penaeidin4, were also induced by rLvCHH I and rLvCHH II. These results demonstrated that host immune response, in addition to glucose metabolism, could be directly modulated by LvCHH family, and the present study provided new insights into the immunomodulation role of metabolic hormones in invertebrate. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordShrimp Wssv Infection Crustacean Hyperglycemic Hormones Glucose Metabolism Immunomodulation
DOI10.1016/j.fsi.2017.01.007
Indexed BySCI
Language英语
WOS IDWOS:000395955800018
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136760
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Experimental Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Cotrol, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Poltech Univ, Dalian 116034, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Lin,Chen, Hao,Xu, Jianchao,et al. Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2017,62:164-174.
APA Wang, Lin.,Chen, Hao.,Xu, Jianchao.,Xu, Qingsong.,Wang, Mengqiang.,...&Song, Linsheng.(2017).Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,62,164-174.
MLA Wang, Lin,et al."Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 62(2017):164-174.
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