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The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus
Li, Yiqun1,3; Song, Xiaorui2; Wang, Weilin1,3; Wang, Lingling2; Yi, Qilin2; Jiang, Shuai1; Jia, Zhihao1,3; Du, Xinyu1; Qiu, Limei1; Song, Linsheng2
2017-06-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume71Pages:59-69
SubtypeArticle
AbstractIncreasing evidences have demonstrated that the invertebrate gill is a predominant tissue participating in the immune response during pathogen challenge. In the present study, the hematopoiesis and immune activities in gill of Pacific oyster Crassostrea gigas were investigated. Stem-like cells with big nuclei and thin cytoplasm were found in the tubules of gill filaments, where DNA synthesis is active and hemocytes production are exuberant. The oysters primarily stimulated by formaldehyde-killed Vibrio splendidus exhibited stronger immune responses and enhanced cell regeneration in gill when they encountered the secondary challenge of live V. splendidus. After the secondary stimulation with V. splendidus, the expression levels of CgClec-4 and CgIFN in the gill of oysters pre-stimulated with formaldehyde-killed V. splendidus were significantly higher (p < 0.05) than that in the oysters pre-stimulated with filter-sterilized (0.22 mu m pore size) sea water, while the expression level of CgIL-17 was significantly decreased (p < 0.05). Meanwhile, the protein expression level of hematopoietic transcription factor CgGATA3 and immune-related protein CgEcSOD in gill increased apparently after the secondary challenge with V. splendidus. ROS production was also enhanced (p < 0.05) at 6 h and 24 h after the secondary challenge. The phagocytic rate in gill of oysters pre-stimulated with formaldehyde-killed V. splendidus was significantly increased (p < 0.05) at 6 h after the secondary challenge with live V. splendidus, showing faster response than that pre-stimulated with filter-sterilized sea water. These results collectively showed that the immune parameters in gill were apparently enhanced after secondary challenge With live V. splendidus, indicating that hematopoiesis might participate in immune priming in Pacific oyster C gigas. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Gill Hematopoiesis Immune Priming Cell Regeneration Enhanced Immune Response
DOI10.1016/j.dci.2017.01.024
Indexed BySCI
Language英语
WOS IDWOS:000397834800007
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136728
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Yiqun,Song, Xiaorui,Wang, Weilin,et al. The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,71:59-69.
APA Li, Yiqun.,Song, Xiaorui.,Wang, Weilin.,Wang, Lingling.,Yi, Qilin.,...&Song, Linsheng.(2017).The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,71,59-69.
MLA Li, Yiqun,et al."The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 71(2017):59-69.
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