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A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish
Li, Xue-peng1,2,3; Sun, Li1,2
2017-06-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume71Pages:49-58
SubtypeArticle
AbstractComplement factor B (Bf) is a component of the complement system. Following activation of the alternative pathway of the complement system, factor B is cleaved into Ba and Bb fragments. In fish, the Bf of rainbow trout is known to act as a C3 convertase, but the function of the Ba fragment is essentially unknown. In this study, we examined the expression patterns of tongue sole Cynoglossus semilaevis Bf (named CsBf) and the biological activity of the Ba fragment of CsBf (named CsBa). CsBf possesses the conserved domains of Bf and shares 39.9%-56.4% sequence identities With other fish Bf. CsBf expression was high in liver, muscle, and heart, and low in intestine, blood, and kidney. Bacterial infection significantly induced CsBf expression in kidney, spleen, and liver in a time-dependent manner. Recombinant CsBa (rCsBa) exhibited apparent binding capacities to bacteria and tongue sole peripheral blood leukocytes, and binding of rCsBa to bacteria inhibited bacterial growth. When overexpressed in tongue sole, CsBa significantly reduced bacterial dissemination in fish tissues. Together these results indicate for the first time that a fish Ba possesses antibacterial effect as well as immune cell-binding capacity, and thus probably plays a role in host immune defense against bacterial infection. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordComplement Factor b Cynoglossus Semilaevis Antimicrobial Bacterial Infection
DOI10.1016/j.dci.2017.01.021
Indexed BySCI
Language英语
WOS IDWOS:000397834800006
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136727
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Xue-peng,Sun, Li. A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,71:49-58.
APA Li, Xue-peng,&Sun, Li.(2017).A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,71,49-58.
MLA Li, Xue-peng,et al."A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 71(2017):49-58.
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