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Enhanced poly(3-hydroxypropionate) production via beta-alanine pathway in recombinant Escherichia coli
Lacmata, Stephen Tamekou1,2; Kuiate, Jules-Roger2; Ding, Yamei3; Xian, Mo1; Liu, Huizhou1; Boudjeko, Thaddee4; Feng, Xinjun1; Zhao, Guang1,5
2017-03-02
Source PublicationPLOS ONE
Volume12Issue:3
SubtypeArticle
AbstractPoly(3-hydroxypropionate) (P3HP) is a thermoplastic with great compostability and biocompatibility, and can be produced through several biosynthetic pathways, in which the glycerol pathway achieved the highest P3HP production. However, exogenous supply of vitamin B-12 was required to maintain the activity of glycerol dehydratase, resulting in high production cost. To avoid the addition of VB12, we have previously constructed a P3HP biosynthetic route with beta-alanine as intermediate, and the present study aimed to improve the P3HP production of this pathway. L-aspartate decarboxylase PanD was found to be the rate-limiting enzyme in the beta-alanine pathway firstly. To improve the pathway efficiency, PanD was screened from four different sources (Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens, and Corynebacterium glutamicum). And PanD from C. glutamicum was found to have the highest activity, the P3HP production was improved in flask cultivation with this enzyme. To further improve the production, the host strain was screened and the culture condition was optimized. Under optimal conditions, production and content of P3HP reached to 10.2 g/L and 39.1% (wt/wt [cell dry weight]) in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without VB12.
DOI10.1371/journal.pone.0173150
Indexed BySCI
Language英语
WOS IDWOS:000396011300081
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136371
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China
2.Univ Dschang, Fac Sci, Dept Biochem, Lab Microbiol & Antimicrobials Subst, Dschang, Cameroon
3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
4.Univ Yaounde, Lab Phytoptotect & Valorizat Vegetals Resources, Biotechnol Nkolbisson Ctr, Dept Biochem, Yaounde, Cameroon
5.Randian Technol Co Ltd, Tianjin, Peoples R China
Recommended Citation
GB/T 7714
Lacmata, Stephen Tamekou,Kuiate, Jules-Roger,Ding, Yamei,et al. Enhanced poly(3-hydroxypropionate) production via beta-alanine pathway in recombinant Escherichia coli[J]. PLOS ONE,2017,12(3).
APA Lacmata, Stephen Tamekou.,Kuiate, Jules-Roger.,Ding, Yamei.,Xian, Mo.,Liu, Huizhou.,...&Zhao, Guang.(2017).Enhanced poly(3-hydroxypropionate) production via beta-alanine pathway in recombinant Escherichia coli.PLOS ONE,12(3).
MLA Lacmata, Stephen Tamekou,et al."Enhanced poly(3-hydroxypropionate) production via beta-alanine pathway in recombinant Escherichia coli".PLOS ONE 12.3(2017).
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