| Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein | |
Wang, Xueqin; Yu, Huahua ; Xing, Ronge; Chen, Xiaolin; Liu, Song; Li, Pengcheng
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| 2017 | |
| 发表期刊 | BIOMED RESEARCH INTERNATIONAL
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| 文章类型 | Article |
| 摘要 | This study optimizes the preparation conditions for mackerel protein hydrolysate (MPH) by response surface methodology (RSM) and investigates the stability of the antioxidant activity of MPHs (<2.5 kDa). The optimal conditions were as follows: enzyme concentration of 1726.85U/g, pH of 7.00, temperature of 39.55 degrees C, time of 5.5 h, and water/material ratio of 25 : 1, and the maximum DPPH scavenging activity was 79.14%. The MPHs indicated significant cellular antioxidant activity at low concentrations. Furthermore, the temperature and freeze-thaw cycles had little effect on the antioxidative stability while pH had significant effect on the antioxidative stability. In addition, the MPHs were sensitive to the metal ions, such as Fe2+, Fe3+, Zn2+, and Cu2+. Notably, when the concentrations of Fe2+ and Fe3+ were 5mM, the DPPH scavenging activities were only 1.1% and 0.6%, respectively; furthermore, Cu2+ at a 5mM concentration could completely inhibit the DPPH scavenging activity of MPHs. In contrast, K+ and Mg2+ had no notable effect on the antioxidant activity of MPHs. These results may provide a scientific basis for the processing and application of MPHs. |
| DOI | 10.1155/2017/6837285 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394006200001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/136337 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein[J]. BIOMED RESEARCH INTERNATIONAL,2017. |
| APA | Wang, Xueqin,Yu, Huahua,Xing, Ronge,Chen, Xiaolin,Liu, Song,&Li, Pengcheng.(2017).Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein.BIOMED RESEARCH INTERNATIONAL. |
| MLA | Wang, Xueqin,et al."Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein".BIOMED RESEARCH INTERNATIONAL (2017). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Optimization of the （3495KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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