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Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein
Wang, Xueqin; Yu, Huahua; Xing, Ronge; Chen, Xiaolin; Liu, Song; Li, Pengcheng
2017
Source PublicationBIOMED RESEARCH INTERNATIONAL
SubtypeArticle
AbstractThis study optimizes the preparation conditions for mackerel protein hydrolysate (MPH) by response surface methodology (RSM) and investigates the stability of the antioxidant activity of MPHs (<2.5 kDa). The optimal conditions were as follows: enzyme concentration of 1726.85U/g, pH of 7.00, temperature of 39.55 degrees C, time of 5.5 h, and water/material ratio of 25 : 1, and the maximum DPPH scavenging activity was 79.14%. The MPHs indicated significant cellular antioxidant activity at low concentrations. Furthermore, the temperature and freeze-thaw cycles had little effect on the antioxidative stability while pH had significant effect on the antioxidative stability. In addition, the MPHs were sensitive to the metal ions, such as Fe2+, Fe3+, Zn2+, and Cu2+. Notably, when the concentrations of Fe2+ and Fe3+ were 5mM, the DPPH scavenging activities were only 1.1% and 0.6%, respectively; furthermore, Cu2+ at a 5mM concentration could completely inhibit the DPPH scavenging activity of MPHs. In contrast, K+ and Mg2+ had no notable effect on the antioxidant activity of MPHs. These results may provide a scientific basis for the processing and application of MPHs.
DOI10.1155/2017/6837285
Indexed BySCI
Language英语
WOS IDWOS:000394006200001
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136337
Collection海洋生物技术研发中心
海洋环境工程技术研究发展中心
AffiliationChinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein[J]. BIOMED RESEARCH INTERNATIONAL,2017.
APA Wang, Xueqin,Yu, Huahua,Xing, Ronge,Chen, Xiaolin,Liu, Song,&Li, Pengcheng.(2017).Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein.BIOMED RESEARCH INTERNATIONAL.
MLA Wang, Xueqin,et al."Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein".BIOMED RESEARCH INTERNATIONAL (2017).
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