IOCAS-IR  > 实验海洋生物学重点实验室
The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri
Jia, Zhihao1,3; Wang, Mengqiang1; Yue, Feng1,3; Wang, Xiudan1,3; Wang, Lingling2; Song, Linsheng2
2017
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume60Pages:141-149
SubtypeArticle
AbstractTranslationally controlled tumor protein (TLi1) is initially described as a highly conserved protein implicated in cell growth, and it is subsequently confirmed to play important roles in mediating the innate immune response, especially the inflammatory. In the present study, the full-length cDNA sequence of a TCTP from Zhikong scallop Chlamys farreri (designed as CfTCTP) was cloned by rapid amplification of cDNA ends (RACE) technique based on the expression sequence tag (EST) analysis. It was of 1230 bp with an open reading frame (ORF) of 543 bp encoding a polypeptide of 180 amino acids. The deduced amino acid sequence contained a conserved TCTP signature sequence (from I-47 to E-58) and it shared 26.1%-48.9% similarities with previously identified TCTPs. CJICTP was clustered with the TCTP from Argopectehs irradias in the phylogenetic tree and was designated into a single branch of mollusk with TCFP from Ruditapes philippinarum. The mRNA transcripts of CfTCTP were constitutively expressed in all the tested tissues, including haemocytes, muscle, mantle, gill, hepatopancreas, kidney and gonad, with the highest expression level in hepatopancreas. The mRNA expression level of CfTCTP in oocytes and fertilized eggs kept at a higher level, and was down-regulated from 2-cell embryos to the lowest level in gastrula. Then it was up-regulated in trochophore and dropped down in the late veliger larvae to the similar level as that in oocytes. After pathogen-associated molecular patterns (PAMPs) stimulation, the expression of CfFCFP mRNA in haemocytes was increased at 3 or 6 h, and fall down to the normal level at 24 h. The recombinant protein of CfTCTP could induce the release of histamine from BT-549 cells. All these results indicated that CfTCTP was a pro-inflammatory factor and it could be maternally transferred from female gonad to oocytes and offspring, and play pivotal role in the embryonic development and immune protection of scallops. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordChlamys Farreri Tctp Maternal Larval Development Innate Immune Response Histamine-releasing
DOI10.1016/j.fsi.2016.11.043
Indexed BySCI
Language英语
WOS IDWOS:000392770000019
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136311
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Zhihao,Wang, Mengqiang,Yue, Feng,et al. The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2017,60:141-149.
APA Jia, Zhihao,Wang, Mengqiang,Yue, Feng,Wang, Xiudan,Wang, Lingling,&Song, Linsheng.(2017).The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,60,141-149.
MLA Jia, Zhihao,et al."The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 60(2017):141-149.
Files in This Item:
File Name/Size DocType Version Access License
The immunomodulation(2369KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jia, Zhihao]'s Articles
[Wang, Mengqiang]'s Articles
[Yue, Feng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jia, Zhihao]'s Articles
[Wang, Mengqiang]'s Articles
[Yue, Feng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jia, Zhihao]'s Articles
[Wang, Mengqiang]'s Articles
[Yue, Feng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.