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The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri
Jia, Zhihao1,3; Wang, Mengqiang1; Yue, Feng1,3; Wang, Xiudan1,3; Wang, Lingling2; Song, Linsheng2
2017
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号60页码:141-149
文章类型Article
摘要Translationally controlled tumor protein (TLi1) is initially described as a highly conserved protein implicated in cell growth, and it is subsequently confirmed to play important roles in mediating the innate immune response, especially the inflammatory. In the present study, the full-length cDNA sequence of a TCTP from Zhikong scallop Chlamys farreri (designed as CfTCTP) was cloned by rapid amplification of cDNA ends (RACE) technique based on the expression sequence tag (EST) analysis. It was of 1230 bp with an open reading frame (ORF) of 543 bp encoding a polypeptide of 180 amino acids. The deduced amino acid sequence contained a conserved TCTP signature sequence (from I-47 to E-58) and it shared 26.1%-48.9% similarities with previously identified TCTPs. CJICTP was clustered with the TCTP from Argopectehs irradias in the phylogenetic tree and was designated into a single branch of mollusk with TCFP from Ruditapes philippinarum. The mRNA transcripts of CfTCTP were constitutively expressed in all the tested tissues, including haemocytes, muscle, mantle, gill, hepatopancreas, kidney and gonad, with the highest expression level in hepatopancreas. The mRNA expression level of CfTCTP in oocytes and fertilized eggs kept at a higher level, and was down-regulated from 2-cell embryos to the lowest level in gastrula. Then it was up-regulated in trochophore and dropped down in the late veliger larvae to the similar level as that in oocytes. After pathogen-associated molecular patterns (PAMPs) stimulation, the expression of CfFCFP mRNA in haemocytes was increased at 3 or 6 h, and fall down to the normal level at 24 h. The recombinant protein of CfTCTP could induce the release of histamine from BT-549 cells. All these results indicated that CfTCTP was a pro-inflammatory factor and it could be maternally transferred from female gonad to oocytes and offspring, and play pivotal role in the embryonic development and immune protection of scallops. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Chlamys Farreri Tctp Maternal Larval Development Innate Immune Response Histamine-releasing
DOI10.1016/j.fsi.2016.11.043
收录类别SCI
语种英语
WOS记录号WOS:000392770000019
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136311
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Jia, Zhihao,Wang, Mengqiang,Yue, Feng,et al. The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2017,60:141-149.
APA Jia, Zhihao,Wang, Mengqiang,Yue, Feng,Wang, Xiudan,Wang, Lingling,&Song, Linsheng.(2017).The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,60,141-149.
MLA Jia, Zhihao,et al."The immunomodulation of a maternal translationally controlled tumor protein (TCTP) in Zhikong scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 60(2017):141-149.
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