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Molecular characterization reveals involvement of four caspases in the antibacterial immunity of tongue sole (Cynoglossus semilaevis)
Long, Hao1,2; Sun, Li1,2
2016-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume57Pages:340-349
SubtypeArticle
AbstractCaspases are a family of proteases involved in many important biological processes including apoptosis and inflammation. In this study, we analyzed in a comparative manner the expression patterns and immune effects of four different types of caspases, i.e. caspase-1 (CsCas1), caspase-2 (CsCas2), caspase-3 (CsCas3), and caspase-9 (CsCas9), from the teleost fish tongue sole (Cynoglossus semilaevis). CsCas1, CsCas2, CsCas3, and CsCas9 share 35.4%-58.5%, 61.2%-75.3%, 523%-65.6%, and 63.0%-76.2% overall sequence identities, respectively, with their counterparts in teleost species. CsCas1, CsCas2, CsCas3, and CsCas9 possess the caspase domain and catalytic site conserved in known caspases. The expressions of the four caspases were detected in a wide range of tissues, however, the expression levels varied between different tissues and caspases. Following bacterial infection, the expressions of the four caspases were upregulated or downregulated to significant extents in a time- and tissue-dependent manner. In vivo analysis showed that overexpression of each of the caspases in tongue sole significantly enhanced the ability of the fish to resist bacterial dissemination in and colonization of tissues. These results indicate an involvement of fish caspases in bacteria-induced immune response and demonstrate for the first time that caspase-1, 2, 3, and 9 are essential to the optimal defense against bacterial infection in fish. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCaspase Cynoglossus Semilaevis Antibacterial Overexpression Apoptosis Edwardsiella Tarda
DOI10.1016/j.fsi.2016.08.047
Indexed BySCI
Language英语
WOS IDWOS:000385327100037
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136157
Collection实验海洋生物学重点实验室
研究生部
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Long, Hao,Sun, Li. Molecular characterization reveals involvement of four caspases in the antibacterial immunity of tongue sole (Cynoglossus semilaevis)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,57:340-349.
APA Long, Hao,&Sun, Li.(2016).Molecular characterization reveals involvement of four caspases in the antibacterial immunity of tongue sole (Cynoglossus semilaevis).FISH & SHELLFISH IMMUNOLOGY,57,340-349.
MLA Long, Hao,et al."Molecular characterization reveals involvement of four caspases in the antibacterial immunity of tongue sole (Cynoglossus semilaevis)".FISH & SHELLFISH IMMUNOLOGY 57(2016):340-349.
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