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Characterization and expression of a novel caspase gene: Evidence of the expansion of caspases in Crassostrea gigas
Li, Zhongxiao1,2; Wang, Chao1; Jiang, Fengjuan1,2; Huan, Pin1; Liu, Baozhong1,3
2016-11-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume201Pages:37-45
SubtypeArticle
AbstractCaspases are a group of cysteine-aspartate proteases involved in apoptosis and a variety of non-apoptotic processes. In this study, a novel caspase gene was cloned and its potential role in apoptosis was investigated. The caspase gene (CgCasp 3/7) has an open reading frame of 1626 bp encoding 541 amino acids containing the conserved functional domains and motifs of effector caspases. Its amino acid sequence shows low identity with the other effector caspases of Crassostrea gigas and contains a unique long intersubunit linker (IL). The CgCasp 3/7 mRNA was expressed highly in oocytes and then decreased gradually after fertilization, indicating CgCasp 3/7 could function in oocyte apoptosis. In adult tissues, it is located primarily in the gills and hepatopancreas. We examined the mRNA expression of CgCasp 3/7 in gills of oysters immersed in ambient (17 degrees C) or heated (27 degrees C) seawater. The thermal stress stimulated mRNA expression of CgCasp 3/7 by 2.5- and 4.1-fold at 2 h and 6 h post-treatment, respectively, indicating CgCasp3/7 was involved in the early response to thermal stress. To examine the function of the IL, CgCasp 3/7 and CgCasp 3/7-T (with a truncated IL) were expressed using an in vitro translation system and their DEVDase activity was measured. Both proteins showed a significantly higher level of DEVDase activity than control, but CgCasp3/7-T had lower DEVDase activity than CgCasp3/7, indicating CgCasp3/7 had DEVDase activity and the IL was required for maximal DEVDase activity. Our study adds to the complexity of caspases in C gigas. (C) 2016 Elsevier Inc. All rights reserved.
KeywordCrassostrea Gigas Caspase Thermal Stress Apoptosis
DOI10.1016/j.cbpb.2016.07.001
Indexed BySCI
Language英语
WOS IDWOS:000385330500005
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136149
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Zhongxiao,Wang, Chao,Jiang, Fengjuan,et al. Characterization and expression of a novel caspase gene: Evidence of the expansion of caspases in Crassostrea gigas[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2016,201:37-45.
APA Li, Zhongxiao,Wang, Chao,Jiang, Fengjuan,Huan, Pin,&Liu, Baozhong.(2016).Characterization and expression of a novel caspase gene: Evidence of the expansion of caspases in Crassostrea gigas.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,201,37-45.
MLA Li, Zhongxiao,et al."Characterization and expression of a novel caspase gene: Evidence of the expansion of caspases in Crassostrea gigas".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 201(2016):37-45.
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