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The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas
Liu, Zhaoqun1,3; Zhou, Zhi1; Wang, Lingling2; Dong, Wenjing1; Qiu, Limei1; Song, Linsheng2
2016-12-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume65Pages:139-148
SubtypeArticle
AbstractMuscarinic receptors, which selectively take muscarine as their ligand, are critical for the immunological and physiological processes in animals. In the present study, the open region frame (ORF) of a homologue of muscarinic acetylcholine (ACh) receptor (mAChR) was amplified from oyster Crassostrea gigas (named as CgmAChR-1), whose full length was 1983 bp and the protein it encoded contained 660 amino acids with a seven transmembrane region. Phylogeny analysis suggested that CgmAChR-1 shared homology with M-5 muscarinic receptor found in invertebrates including Habropoda laboriosa, Acromyrmex echinatior and Echinococcus granulosus. After cell transfection of CgrnAChR-1 into HEK293T cells and ACh incubation, the level of intracellular Ca2+ and CAMP increased significantly (p < 0.05). Such trend could be reverted with the addition of M-3 and M-5 muscarinic receptor antagonists DAMP and DAR. The CgmAChR-1 transcripts were ubiquitously detectable in seven different tissues with the maximal expression level in adductor muscle. When the oysters received LPS stimulation, CgmAChR-1 mRNA expression in haemocyte was increased to the highest level (6.05-fold, p < 0.05) at 24 h, while blocking CgmAChR-1 using receptor antagonists before LPS stimulation promoted the expression of oyster TNF, resulting in the increase of haemocyte apoptosis index. These results suggested that CgmAChR-1 was the key molecule in cholinergic neuroendocrine-immune system contributing to the regulation of TNF expression and apoptosis process. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Acetylcholine Muscarinic Acetylcholine Receptor Tnf Pathway Immune Regulation
DOI10.1016/j.dci.2016.07.003
Indexed BySCI
Language英语
WOS IDWOS:000384388400016
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135892
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,et al. The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:139-148.
APA Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,Dong, Wenjing,Qiu, Limei,&Song, Linsheng.(2016).The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,139-148.
MLA Liu, Zhaoqun,et al."The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):139-148.
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