IOCAS-IR  > 实验海洋生物学重点实验室
The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas
Liu, Zhaoqun1,3; Zhou, Zhi1; Wang, Lingling2; Dong, Wenjing1; Qiu, Limei1; Song, Linsheng2
2016-12-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号65页码:139-148
文章类型Article
摘要Muscarinic receptors, which selectively take muscarine as their ligand, are critical for the immunological and physiological processes in animals. In the present study, the open region frame (ORF) of a homologue of muscarinic acetylcholine (ACh) receptor (mAChR) was amplified from oyster Crassostrea gigas (named as CgmAChR-1), whose full length was 1983 bp and the protein it encoded contained 660 amino acids with a seven transmembrane region. Phylogeny analysis suggested that CgmAChR-1 shared homology with M-5 muscarinic receptor found in invertebrates including Habropoda laboriosa, Acromyrmex echinatior and Echinococcus granulosus. After cell transfection of CgrnAChR-1 into HEK293T cells and ACh incubation, the level of intracellular Ca2+ and CAMP increased significantly (p < 0.05). Such trend could be reverted with the addition of M-3 and M-5 muscarinic receptor antagonists DAMP and DAR. The CgmAChR-1 transcripts were ubiquitously detectable in seven different tissues with the maximal expression level in adductor muscle. When the oysters received LPS stimulation, CgmAChR-1 mRNA expression in haemocyte was increased to the highest level (6.05-fold, p < 0.05) at 24 h, while blocking CgmAChR-1 using receptor antagonists before LPS stimulation promoted the expression of oyster TNF, resulting in the increase of haemocyte apoptosis index. These results suggested that CgmAChR-1 was the key molecule in cholinergic neuroendocrine-immune system contributing to the regulation of TNF expression and apoptosis process. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Crassostrea Gigas Acetylcholine Muscarinic Acetylcholine Receptor Tnf Pathway Immune Regulation
DOI10.1016/j.dci.2016.07.003
收录类别SCI
语种英语
WOS记录号WOS:000384388400016
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/135892
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,et al. The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:139-148.
APA Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,Dong, Wenjing,Qiu, Limei,&Song, Linsheng.(2016).The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,139-148.
MLA Liu, Zhaoqun,et al."The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):139-148.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
The cholinergic immu(2774KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Zhaoqun]的文章
[Zhou, Zhi]的文章
[Wang, Lingling]的文章
百度学术
百度学术中相似的文章
[Liu, Zhaoqun]的文章
[Zhou, Zhi]的文章
[Wang, Lingling]的文章
必应学术
必应学术中相似的文章
[Liu, Zhaoqun]的文章
[Zhou, Zhi]的文章
[Wang, Lingling]的文章
相关权益政策
暂无数据
收藏/分享
文件名: The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。