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Characterization of two pathogenic Photobacterium strains isolated from Exopalaemon carinicauda causing mortality of shrimp
Liu, Fei1,2; Liu, Guangxing1; Li, Fuhua2,3
2016-11-01
Source PublicationAQUACULTURE
Volume464Pages:129-135
SubtypeArticle
AbstractBacterial and viral infectious diseases are serious problems in shrimp farming. During the culture of Exopalaemon carinicauda, we found that some individuals showed typical symptoms with pale color for their hepatopancreas, and whitish color for their body, which caused the final mortality of shrimp. In order to know the agent which caused the mortality of shrimp, we isolated the dominant bacteria and analyzed their characteristics. Histopathological observation showed that the tubule lumens of hepatopancreas were enlarged, and the cells located at the hepatopancreatic tubes were damaged severely. Two dominant strains named WH01 and WH02 were isolated from the hepatopancreas of diseased shrimp by thiosulfate citrate bile salt sucrose (TCBS) agar. Analysis on their partial 16S rRNA sequences showed that WH01 and WH02 had the highest similarity with Photobacterium damselae subsp. damselae. Furthermore, a specific molecular methodology (using toxR gene) and Biolog system phenotypic analysis confirmed that WH01 and WH02 were two strains of Photobacterium damselae subsp. damselae. Hemolysis test showed that both WH01 and WH02 strains could cause beta hemolysis on sheep blood agar plates. Artificial infection test revealed that both WH01 and WH02 could infect the shrimp E. carinicauda and lead to 100% mortality. The median lethal dose (LD50) for WH01 was 2.30 x 10(5) CFU/g (body weight) and the LD50 for WH02 was 1.09 x 10(5) CFU/g (body weight). In addition, these two strains could also infect the shrimp Litopenaeus vannamei to cause its mortality by intramuscular injection. These data indicated that Photobacterium might be a common pathogen of shrimps. The pathogenicity of Photobacterium to shrimp must be paid more attention for shrimp farming. (C) 2016 Elsevier B.V. All rights reserved.
KeywordPhotobacterium Pathogenicity Identification Exopalaemon Carinicauda
DOI10.1016/j.aquaculture.2016.06.019
Indexed BySCI
Language英语
WOS IDWOS:000383370300018
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135891
Collection实验海洋生物学重点实验室
Affiliation1.Ocean Univ China, Key Lab Marine Environm & Ecol, Minist Educ, Qingdao 266100, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Fei,Liu, Guangxing,Li, Fuhua. Characterization of two pathogenic Photobacterium strains isolated from Exopalaemon carinicauda causing mortality of shrimp[J]. AQUACULTURE,2016,464:129-135.
APA Liu, Fei,Liu, Guangxing,&Li, Fuhua.(2016).Characterization of two pathogenic Photobacterium strains isolated from Exopalaemon carinicauda causing mortality of shrimp.AQUACULTURE,464,129-135.
MLA Liu, Fei,et al."Characterization of two pathogenic Photobacterium strains isolated from Exopalaemon carinicauda causing mortality of shrimp".AQUACULTURE 464(2016):129-135.
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