CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects

doi:10.1016/j.fsi.2016.06.055

IOCAS-IR > 实验海洋生物学重点实验室

	CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects
	Wang, Ting1,2,3 ; Zhang, Jian 1,2
	2016-09-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	56 页码:12-20
文章类型	Article
摘要	Pentraxin 1 (PTX1) is a member of the pentraxin protein family, which plays important roles in the innate immunity of vertebrates. In fish, the biological function of PTX1 is essentially unknown. In this study, we examined the expression and function of a PTX homologue (CsPTX1) from the tongue sole, Cynoglossus semilaevis. CsPTX1 contains 223 amino acids and shares 49.3%-38.8% overall sequence identity with other known fish pentraxins. CsPTX1 is expressed in multiple tissues and is upregulated by bacterial and viral infection. CsPTX1 contains a pentraxin domain, which is known to bind extracellular antigens, and recombinant CsPTX1 (rCsPTX1) bound a wide range of Gram-positive and Gram-negative bacteria. rCsPTX1 also agglutinated all the bacteria tested in a Ca2+-dependent manner and the agglutinating capacity of rCsPTX1 was abolished in the absence of calcium. As well as its ability to agglutinate bacterial cells, rCsPTX1 displayed apparent bacteriostatic activity against Pseudomonas fluorescens in vitro by influencing the permeability of the microbial envelope. When introduced in vivo, rCsPTX1 enhanced the host's resistance to bacterial infection. These results indicate that CsPTX1 is a classic pattern recognition molecule that defends C semilaevis against bacterial infection. (C) 2016 Elsevier Ltd. All rights reserved.
关键词	Pentraxin Cynoglossus Semilaevis Innate Immunity Bacterial Agglutination Antibacterial Effect
DOI	10.1016/j.fsi.2016.06.055
收录类别	SCI
语种	英语
WOS记录号	WOS:000383292200002
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/135839
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Ting,Zhang, Jian. CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:12-20.
APA	Wang, Ting,&Zhang, Jian.(2016).CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects.FISH & SHELLFISH IMMUNOLOGY,56,12-20.
MLA	Wang, Ting,et al."CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects".FISH & SHELLFISH IMMUNOLOGY 56(2016):12-20.

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