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Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus
Ji Liqin1,2; Jiang Keyong1; Liu Mei1; Wang Baojie1; Han Longjiang1,3; Zhang Mingming1,3; Wang Lei1
2016-05-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume34Issue:3Pages:430-440
SubtypeArticle
AbstractTo study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot (Scophthalmus maximus), water temperature was lowered rapidly from 18 to 1 degrees C. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1 degrees C. Blood samples and tissues were taken from each individual, hematological indices and HSP gene expression in tissues were measured. The red blood cell count, white blood cell count, and hemoglobin concentration decreased significantly (P < 0.05) as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased, whereas aspartic transaminase and gamma-glutamyl transpeptidase activities displayed no obvious changes above 1 degrees C and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8 degrees C, and creatinine concentration was highest at 3 degrees C. The concentrations of plasma cortisol, cholesterol, and triglyceride all increased significantly (P < 0.05) as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues, whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall, these results indicate that temperature decreases in the range of 8 to 5 degrees C may induce a stress response in S. maximus and that temperature should be kept above 8 degrees C in the aquaculture setting to avoid damage to the fish.
KeywordHematology Hsp70 Hsp90 Mrna Expression Low Temperature Stress Fish
DOI10.1007/s00343-016-4367-z
Indexed BySCI
Language英语
WOS IDWOS:000375677600002
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131069
Collection海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China
Recommended Citation
GB/T 7714
Ji Liqin,Jiang Keyong,Liu Mei,et al. Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(3):430-440.
APA Ji Liqin.,Jiang Keyong.,Liu Mei.,Wang Baojie.,Han Longjiang.,...&Wang Lei.(2016).Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(3),430-440.
MLA Ji Liqin,et al."Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.3(2016):430-440.
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