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The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas
Xin, Lusheng1,3; Wang, Mengqiang1; Zhang, Huan1; Li, Meijia1,3; Wang, Hao1; Wang, Lingling1; Song, Linsheng2
2016-07-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume54Pages:118-127
SubtypeArticle
AbstractMyD88 serves as a critical cytosolic adaptor mediating activation of NF-kappa B in innate immunity. It has been found that there is a considerable expansion of MyD88 in Crassostrea gigas. In the present study, four typical MyD88 genes in Crassostrea gigas (CgMyD88-A to CgMyD88-D) were successfully cloned and their potential functions were investigated together with another two known ones (CgMyD88-T1 and CgMyD88-T2). Multiple alignments revealed that CgMyD88-B and CgMyD88-C remained the conserved DD and TIR domains, while there was a significant variation of E51Q in the DD of CgMyD88-A, and some variations in both DD and TIR domains of CgMyD88-D, respectively. Both truncated CgMyD88-T1 and CgMyD88-T2 lacked Box II in their only TIR domains. Expression pattern analysis showed that CgMyD88-B and CgMyD88-C genes possessed higher expression in normal tissues, compared with the other four. When oysters were under bacteria challenge, CgMyD88-B, CgMyD88-C, CgMyD88-T1 and CgMyD88-T2 were firstly induced, while CgMyD88-A and CgMyD88-D were suppressed. Dual luciferase reporter assays showed that CgMyD88-B and CgMyD88-C could promote the activation of NF-kappa B signaling pathway, while the other four CgMyD88 genes failed or even suppressed the activities of CgMyD88-B and CgMyD88-C on the activation of NF-kappa B signaling. It was deduced that after oysters were challenged by bacteria, CgMyD88-B and CgMyD88-C could rapidly and efficiently activate NF-kappa B signaling pathway to elicit anti-pathogen responses before suppressor CgMyD88 genes (CgMyD88-T1 and CgMyD88-T2) exceeding their expression level. These results suggested that there was mutual modulation of expanded CgMyD88 genes on activating NE-kappa B signaling pathway in oyster C. gigas. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Myd88 Innate Immunity Death Domain Tir Domain Nf-kappa b
DOI10.1016/j.fsi.2016.04.014
Indexed BySCI
Language英语
WOS IDWOS:000378451700014
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130995
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture Stock Enhancement North China, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
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Xin, Lusheng,Wang, Mengqiang,Zhang, Huan,et al. The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:118-127.
APA Xin, Lusheng.,Wang, Mengqiang.,Zhang, Huan.,Li, Meijia.,Wang, Hao.,...&Song, Linsheng.(2016).The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,54,118-127.
MLA Xin, Lusheng,et al."The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 54(2016):118-127.
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