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| An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2 | |
Chen, Hao1,2 ; Zhou, Zhi1; Wang, Hao1; Wang, Lingling3; Wang, Weilin1,2; Liu, Rui1; Qiu, Limei1; Song, Linsheng3
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| 2016-07-12 | |
| 发表期刊 | SCIENTIFIC REPORTS
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| 卷号 | 6 |
| 文章类型 | Article |
| 摘要 | Nuclear factor (NF)-kappa B pathway is an evolutionally conserved pathway in activating immune response, in which I kappa Bs can repress the activation. In the present study, cgi-miR-2d, an invertebrate-specific microRNA, was proved to regulate CgI kappa B2 expression and haemocyte phagocytosis during bacterial infection in oyster Crassostrea gigas. The expression of cgi-miR-2d was significantly up-regulated after Vibrio splendidus challenge, while CgI kappa B2 transcripts decreased. Significant decreases in both luminescence and CgI kappa B2 3'UTR level was observed after transfection of cgi-miR-2d in CgI kappa B2 3'UTR luciferase reporter assay. CgI kappa B2 mRNA level decreased significantly (0.51-fold of control group, p < 0.05) in gain-of-function assay of cgi-miR-2d in vivo while it increased markedly (1.27-fold, p < 0.05) when cgi-miR-2d was repressed (0.10-fold, p < 0.01). A significant increase of haemocyte phagocytosis rate was observed in cgi-miR-2d overexpression group (p < 0.01), consistent with results in CgI kappa B2 knock-down group (p < 0.01). Moreover, the apoptosis rate of haemocytes was found significantly declined (28.57%, p < 0.01) in gain-of-function assay of cgi-miR-2d. Together, those results not only depicted the functional conservation of miR-2d family in anti-apoptosis of oysters but also highlighted its interaction with phagocytosis by modulating NF-kappa B pathway, which might dedicate critically to the well-balance of host immune response. |
| DOI | 10.1038/srep29591 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000379390900001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/130961 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Chen, Hao,Zhou, Zhi,Wang, Hao,et al. An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Chen, Hao.,Zhou, Zhi.,Wang, Hao.,Wang, Lingling.,Wang, Weilin.,...&Song, Linsheng.(2016).An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2.SCIENTIFIC REPORTS,6. |
| MLA | Chen, Hao,et al."An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2".SCIENTIFIC REPORTS 6(2016). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| An invertebrate-spec（1082KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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