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An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2
Chen, Hao1,2; Zhou, Zhi1; Wang, Hao1; Wang, Lingling3; Wang, Weilin1,2; Liu, Rui1; Qiu, Limei1; Song, Linsheng3
2016-07-12
Source PublicationSCIENTIFIC REPORTS
Volume6
SubtypeArticle
AbstractNuclear factor (NF)-kappa B pathway is an evolutionally conserved pathway in activating immune response, in which I kappa Bs can repress the activation. In the present study, cgi-miR-2d, an invertebrate-specific microRNA, was proved to regulate CgI kappa B2 expression and haemocyte phagocytosis during bacterial infection in oyster Crassostrea gigas. The expression of cgi-miR-2d was significantly up-regulated after Vibrio splendidus challenge, while CgI kappa B2 transcripts decreased. Significant decreases in both luminescence and CgI kappa B2 3'UTR level was observed after transfection of cgi-miR-2d in CgI kappa B2 3'UTR luciferase reporter assay. CgI kappa B2 mRNA level decreased significantly (0.51-fold of control group, p < 0.05) in gain-of-function assay of cgi-miR-2d in vivo while it increased markedly (1.27-fold, p < 0.05) when cgi-miR-2d was repressed (0.10-fold, p < 0.01). A significant increase of haemocyte phagocytosis rate was observed in cgi-miR-2d overexpression group (p < 0.01), consistent with results in CgI kappa B2 knock-down group (p < 0.01). Moreover, the apoptosis rate of haemocytes was found significantly declined (28.57%, p < 0.01) in gain-of-function assay of cgi-miR-2d. Together, those results not only depicted the functional conservation of miR-2d family in anti-apoptosis of oysters but also highlighted its interaction with phagocytosis by modulating NF-kappa B pathway, which might dedicate critically to the well-balance of host immune response.
DOI10.1038/srep29591
Indexed BySCI
Language英语
WOS IDWOS:000379390900001
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130961
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Hao,Zhou, Zhi,Wang, Hao,et al. An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2[J]. SCIENTIFIC REPORTS,2016,6.
APA Chen, Hao.,Zhou, Zhi.,Wang, Hao.,Wang, Lingling.,Wang, Weilin.,...&Song, Linsheng.(2016).An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2.SCIENTIFIC REPORTS,6.
MLA Chen, Hao,et al."An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2".SCIENTIFIC REPORTS 6(2016).
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