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Flow cytometric analysis of DNA content for four commercially important crabs in China
Liu Lei1; Cui Zhaoxia2; Song Chengwen2; Liu Yuan2; Hui Min2; Wang Chunlin1
2016-06-01
Source PublicationACTA OCEANOLOGICA SINICA
Volume35Issue:6Pages:7-11
SubtypeArticle
AbstractThe genome size (C-value) of an organism is referring to the DNA content of its non-replicated haploid chromosome complement, generally deduced from measuring somatic diploid nuclei. We presented genome size (C-value) data obtained by flow cytometry for four commercially important crabs (Portunus trituberculatus, Charybdis japonica, Scylla paramamosain, and Eriocheir sinensis) common in the coast of China. Gallus domesticus (2C=2.5 pg ) was used as the internal standard. The results showed that the C-value for P. trituberculatus, C. japonica, S. paramamosain, and E. sinensis were (2.31 +/- 0.01) pg, (2.33 +/- 0.03) pg, (1.64 +/- 0.02) pg, and (2.29 +/- 0.03) pg, respectively. The C-value of P. trituberculatus, C. japonica and S. paramamosain were reported for the first time. The data represented by the four species indicated that they had lower DNA contents than average DNA values in crustaceans ((4.99 +/- 0.48) pg), and three of the four values were very similar if not identical. The results provide useful data for future studies in the fields of biodiversity, species conservation, and phylogeny of these commercial crabs. They will also be helpful in instructing the hybridization breeding program and estimating the cost of the whole genome sequencing project.
KeywordGenome Size Flow Cytometry Crabs Portunidae Grapsidae
DOI10.1007/s13131-016-0876-z
Indexed BySCI
Language英语
WOS IDWOS:000377347900002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/126238
Collection实验海洋生物学重点实验室
Affiliation1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Liu Lei,Cui Zhaoxia,Song Chengwen,et al. Flow cytometric analysis of DNA content for four commercially important crabs in China[J]. ACTA OCEANOLOGICA SINICA,2016,35(6):7-11.
APA Liu Lei,Cui Zhaoxia,Song Chengwen,Liu Yuan,Hui Min,&Wang Chunlin.(2016).Flow cytometric analysis of DNA content for four commercially important crabs in China.ACTA OCEANOLOGICA SINICA,35(6),7-11.
MLA Liu Lei,et al."Flow cytometric analysis of DNA content for four commercially important crabs in China".ACTA OCEANOLOGICA SINICA 35.6(2016):7-11.
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