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A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release
Liu, Conghui1,3; Jiang, Shuai1; Wang, Mengqiang1; Wang, Lingling1; Chen, Hao1,3; Xu, Jiachao1,3; Lv, Zhao1,3; Song, Linsheng1,2
2016-08-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume61Pages:136-144
SubtypeArticle
AbstractSialic acid binding immunoglobulin-type lectin (siglec) belongs to the immunoglobulin superfamily (IgSF), which acts as regulator involved in glycan recognition and signal transduction in the immune and nervous systems. In the present study, a siglec gene (designated CgSiglec-1) was characterized from the Pacific oyster, Crassostrea gigas. The cDNA of CgSiglec-1 was of 1251 bp encoding a predicted polypeptide of 416 amino acids. CgSiglec-1 was composed of two I-set immunoglobulin (Ig) domains, one trans membrane (TM) domain and two ITIM motifs, sharing a sequence similarity with vertebrate CD22 homologs. The mRNA expression of CgSiglec-1 could be detected in all the selected tissues, with the highest level in hemocytes and labial palps. The confocal analysis revealed that CgSiglec-1 mainly distributed on the cytoplasmic membrane of the oyster hemocytes. In addition, the mRNA transcripts of CgSiglec-1 in hemocytes increased significantly (4.29-fold to that of control group, p < 0.05) after Vibrio splendidus stimulation. The recombinant CgSiglec-1 protein (rCgSiglec-1) could bind to poly sialic acid (pSIAS), lipopolysaccharides (LPS) and peptidoglycan (PGN) in a dose-dependent manner. The blockade of CgSiglec-1 by specific polyclonal antibodies could enhance the LPS-induced cell apoptosis, phagocytosis towards V. splendidus and the release of cytokines, such as CgTNF-1, CgIFNLP and CgIL-17. The results collectively indicated that CgSiglec-1 could act as a bridge molecule between invader recognition and signal transduction cascade, and modulate the immune response by inhibiting various important processes of immunity in oyster. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordSialic Acid Binding Immunoglobulin-type Lectins Innate Immune Response Recognition Spectrum Cascading Inhibitory Phagocytosis
DOI10.1016/j.dci.2016.03.026
Indexed BySCI
Language英语
WOS IDWOS:000376804300015
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/126230
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Conghui,Jiang, Shuai,Wang, Mengqiang,et al. A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,61:136-144.
APA Liu, Conghui.,Jiang, Shuai.,Wang, Mengqiang.,Wang, Lingling.,Chen, Hao.,...&Song, Linsheng.(2016).A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,61,136-144.
MLA Liu, Conghui,et al."A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 61(2016):136-144.
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