| Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting | |
Zhao, Feng1,2; Xu, Kuidong1 ; He, Yingying1,2; Xu, KD (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2012-03-01 | |
| 发表期刊 | EUROPEAN JOURNAL OF SOIL BIOLOGY
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| ISSN | 1164-5563 |
| 卷号 | 49页码:112-118 |
| 文章类型 | Article |
| 摘要 | Soil ciliates have advantages in monitoring soil quality after pollution and disturbance. However the potential is limited by the shortcoming of integrating quantitative and qualitative techniques. Here we utilized the Ludox-Quantitative Protargol Stain (Ludox-QPS) method which was originally developed for marine sediment to assess ciliate diversity in soil. The method was tested with three soil samples and compared with a ciliate-specific Denaturing Gradient Gel Electrophoresis (DGGE) and direct count method. The Ludox-QPS method obtained significantly higher number of ciliates with higher taxonomic resolution than the direct count, and revealed higher diversity than the DGGE as well as subsequent gene sequencing. Besides, we evaluated the effects of 2% glutaraldehyde fixative and the QPS preparation on cell size of one species from each of the three dominant ciliate assemblages in soil: colpodids, hypotrichs and haptorids. The experiment indicates a biovolume conversion factor of similar to 0.69 for soil ciliates treated with the Ludox-QPS method. The Ludox-QPS method fulfills the enumeration of soil ciliates with good taxonomic resolution and thus promotes the utilization of ciliates in the bioassessment of soil environments. (C) 2011 Elsevier Masson SAS. All rights reserved. |
| 关键词 | Bioindicator Dgge Direct Count Method Ludox Density Gradient Centrifugation Sequencing |
| 学科领域 | Ecology ; Soil Science |
| DOI | 10.1016/j.ejsobi.2011.11.002 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302736500016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12525 |
| 专题 | 海洋生物分类与系统演化实验室 |
| 通讯作者 | Xu, KD (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Feng,Xu, Kuidong,He, Yingying,et al. Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting[J]. EUROPEAN JOURNAL OF SOIL BIOLOGY,2012,49:112-118. |
| APA | Zhao, Feng,Xu, Kuidong,He, Yingying,&Xu, KD .(2012).Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting.EUROPEAN JOURNAL OF SOIL BIOLOGY,49,112-118. |
| MLA | Zhao, Feng,et al."Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting".EUROPEAN JOURNAL OF SOIL BIOLOGY 49(2012):112-118. |
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