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| Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS) | |
Jin, Weihua1,2; Wang, Jing1 ; Ren, Sumei3; Song, Ni3; Zhang, Quanbin1; Zhang, QB (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
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| 2012-10-01 | |
| 发表期刊 | MARINE DRUGS
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| ISSN | 1660-3397 |
| 卷号 | 10期号:10页码:2138-2152 |
| 文章类型 | Article |
| 摘要 | A fucoidan extracted from Saccharina japonica was fractionated by anion exchange chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and gel filtration chromatography while Fraction Y2 was fractionated by gel filtration chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1 -> 3 Man 1 -> 4 GlcA, Man 1 -> 3 GlcA 1 -> 4 GlcA, Fuc 1 -> 4 GlcA and Fuc 1 -> 3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6. |
| 关键词 | Esi-cid-ms/ms Fucoidan Oligosaccharide Saccharina Japonica |
| 学科领域 | Chemistry, Medicinal |
| DOI | 10.3390/md10102138 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310453600001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12386 |
| 专题 | 实验海洋生物学重点实验室 海洋生物技术研发中心 |
| 通讯作者 | Zhang, QB (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Coll Med & Pharmaceut, Qingdao 266003, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Jin, Weihua,Wang, Jing,Ren, Sumei,et al. Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)[J]. MARINE DRUGS,2012,10(10):2138-2152. |
| APA | Jin, Weihua,Wang, Jing,Ren, Sumei,Song, Ni,Zhang, Quanbin,&Zhang, QB .(2012).Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS).MARINE DRUGS,10(10),2138-2152. |
| MLA | Jin, Weihua,et al."Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)".MARINE DRUGS 10.10(2012):2138-2152. |
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