Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES | |
| Wu, Xiaojie2; Niu, Jianfeng1; Huang, Aiyou1,3; Xu, Meiling2; Wang, Guangce1,2 | |
| 2012-08-01 | |
| 发表期刊 | JOURNAL OF PHYCOLOGY
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| ISSN | 0022-3646 |
| 卷号 | 48期号:4页码:1040-1044 |
| 摘要 | Accurate gene quantification depends on the use of an appropriate internal control gene, which should be verified before its use for normalizing data. Housekeeping genes, which are expressed at relatively constant levels, are generally regarded as candidate internal control genes. To determine the ideal internal control for gene expression profiles for Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) at different life-history stages, we used absolute quantification to assess the expression levels of six housekeeping genes (18S ribosomal RNA, 30S ribosomal protein, glyceraldehyde-3-phosphate dehydrogenase, elongation factor 3, alpha-tubulin, and beta-tubulin) at the sporophyte and gametophyte stages. Housekeeping genes were selected by comparing the differences of observed copy numbers in sporophytes and in gametophytes. TubB (beta-tubulin) was found to be the optimal internal control gene, because it showed the smallest difference of gene expression. Compared with TubB, other housekeeping genes had greater variation of expression to different degrees. |
| 关键词 | absolute quantification internal control gene life-history stage Porphyra haitanensis Rhodophyta |
| DOI | 10.1111/j.1529-8817.2012.01188.x |
| 语种 | 英语 |
| 资助项目 | 973 Project[2011CB411908] ; National Natural Science Foundation of China[30830015] ; National Natural Science Foundation of China[41176134] ; National Natural Science Foundation of China[30970302] ; National Marine Public Welfare Research Project[201105008-2] ; National Marine Public Welfare Research Project[201105023-7] ; Prospective Joint Research Project of Jiangsu Province[BY2011188] |
| WOS研究方向 | Plant Sciences ; Marine & Freshwater Biology |
| WOS类目 | Plant Sciences ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000307016700021 |
| 出版者 | WILEY-BLACKWELL |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12378 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wu, Xiaojie,Niu, Jianfeng,Huang, Aiyou,et al. SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES[J]. JOURNAL OF PHYCOLOGY,2012,48(4):1040-1044. |
| APA | Wu, Xiaojie,Niu, Jianfeng,Huang, Aiyou,Xu, Meiling,&Wang, Guangce.(2012).SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES.JOURNAL OF PHYCOLOGY,48(4),1040-1044. |
| MLA | Wu, Xiaojie,et al."SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES".JOURNAL OF PHYCOLOGY 48.4(2012):1040-1044. |
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