Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5

doi:10.1007/s00343-012-1132-9

IOCAS-IR > 实验海洋生物学重点实验室

	Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5
	Cai Jinling 2; Wei Ying2 ; Zhao Yupeng 2; Pan Guanghua 2; Wang Guangce 1; Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2012-07-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	30 期号:4 页码:620-626
文章类型	Article
摘要	The effects of different NaCl concentrations, nitrogen sources, carbon sources, and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate (PHB) production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%-3% NaCl, suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test, the maximum biomass accumulation (8.10 +/- 0.09 g/L) and PHB production (1.11 +/- 0.13 g/L and 14.62%+/- 2.2 of the cell dry weight) were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH (4) (+) -N was better for PHB production than other nitrogen sources. In the carbon source test, the maximum biomass concentration (7.65 +/- 0.05 g/L) was obtained with malic acid as the sole carbon source, whereas the maximum yield of PHB (5.03 +/- 0.18 g/L and 66.93%+/- 1.69% of the cell dry weight) was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test, sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources, respectively. The best carbon to nitrogen molar ratio for biomass accumulation (8.77 +/- 0.58 g/L) and PHB production (6.07 +/- 0.25 g/L and 69.25%+/- 2.05% of the cell dry weight) was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield.
关键词	Polyhydroxybutyrate Rhodovulum Sulfidophilum Nitrogen Source Carbon Source Carbon To Nitrogen Molar Ratio
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-012-1132-9
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000305906700014
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12372
专题	实验海洋生物学重点实验室
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Cai Jinling,Wei Ying,Zhao Yupeng,et al. Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(4):620-626.
APA	Cai Jinling,Wei Ying,Zhao Yupeng,Pan Guanghua,Wang Guangce,&Wang, GC .(2012).Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(4),620-626.
MLA	Cai Jinling,et al."Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.4(2012):620-626.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Production of polyhy（460KB）			限制开放	使用许可	浏览