Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions

doi:10.1515/bot-2011-0059

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions
	Zhang, Bao Yu 1; Jia, Zhao Jun 2; He, Lin Wen 1; Wang, Guang Ce 1; Peng, Guang 1; Wang, GC (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China.
	2012-08-01
发表期刊	BOTANICA MARINA
ISSN	0006-8055
卷号	55 期号:4 页码:427-434
文章类型	Article
摘要	To characterize the structure and function of the protein phosphatase 2C (PP2C) gene in Porphyra yezoensis, full-length cDNA of the locus (denoted pypp2c) was obtained by electronic cloning, rapid amplification of cDNA ends, and reverse-transcription PCR. The nucleotide sequence of PyPP2C was 1988 bp, including a 5'-untranslated region (UTR) of 301 bp, a 3'-UTR of 256 bp, and an open reading frame of 1431 bp that can be translated into 476 amino acids with a molecular mass of 50.96 kDa and a putative isoelectric point of 8.34. The amino acids had 30-36% identity with those encoded by PP2C genes in protozoa and higher plants. A PP2C-like catalytic domain was found in the 57-397 region, and several residues involved in the metal ion binding sites were conserved. mRNA expression levels in the three life stages of leafy thalli, filaments, and conchospores were examined by real-time fluorescent quantitative PCR. Highest expression of pypp2c occurred in conchospores. Gene expression levels were similar among the three life stages. pypp2c expression at different thallus relative water contents (RWCs) varied, with the highest expression occurring at 60% RWC.
关键词	Desiccation Mrna Expression Level Porphyra Yezoensis Protein Phosphatase 2c Gene Qpcr
学科领域	Plant Sciences ; Marine & Freshwater Biology
DOI	10.1515/bot-2011-0059
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000307499500009
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12299
专题	实验海洋生物学重点实验室
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Bao Yu,Jia, Zhao Jun,He, Lin Wen,et al. Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions[J]. BOTANICA MARINA,2012,55(4):427-434.
APA	Zhang, Bao Yu,Jia, Zhao Jun,He, Lin Wen,Wang, Guang Ce,Peng, Guang,&Wang, GC .(2012).Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions.BOTANICA MARINA,55(4),427-434.
MLA	Zhang, Bao Yu,et al."Characterization of the protein phosphatase 2c gene from Porphyra yezoensis and functional analysis under dessicating conditions".BOTANICA MARINA 55.4(2012):427-434.

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