IOCAS-IR  > 海洋生物分类与系统演化实验室
Thesis Advisor李新正
Degree Grantor中国科学院大学
Place of Conferral北京
Degree Discipline海洋生物学
Keyword深海 海绵 六放海绵 寻常海绵 分类学 系统发育
本论文主要采用传统的形态学方法,以于2013 年到2015 年采集于南海,冲
生境。本研究共收录西太平洋深海海绵2 纲,6 目,17 科,21 属,24 种,包括
2 个新属,10 个新种,其中南海报道了7 种,冲绳海槽报道了7 种,雅浦海山
报道了10 种。在这些种中,有三个种的海绵样品在采集时已经死亡,由于缺乏
本研究还对各实验材料进行了DNA 提取,获得了21 个种的16S rDNA 基因
化关系提供了分子生物学数据。在海绵中运用较普遍的分子标记有18S rDNA,
28S rDNA 和COI 基因,但不同分类阶元运用的引物不同,目前海绵不具有通用
的引物,本文发现运用引物16S1fw/16SH mod 对文中描述的六放海绵纲和寻常
海绵纲的种能较高效地获得其16S rDNA 基因片段,对于是否能高效地用于扩增
Other Abstract
Porifera can be easily found in deep ocean. Morphological plasticity and limited
information obtained from spicules and skeletons make deep-sea sponge classification
very difficult. The sponge taxonomy studies in China are very limited, there are
almost no recodes for deep sea sponge in China. This study performed taxonomic
studies for deep sea sponges, provided valuable information for their biodiversity and
In the study of this thesie, traditional morphological method was used to classify
the sponge samples. The study samples were collected respectively from the South
China Sea, Okinawa Trough and Yap area, West Pacific Ocean, during 2013 to 2015.
They were found from the environment from seamounts, hydrothermal vents, cold
seeps and other deep-sea areas. After sorting the samples, 24 sponges species
belonging to 2 classes, 6 orders, 17 families, 21 genera were identified, in which 2
genera, 10 species were new to science. Among the 24 species, 7, 7, 10 were found
from the South China sea, Okinawa Trough and Yap area respectively. In the samples,
3 specimens were dead when they wre collected, it was too hard to identify them into
species level, however, it is clear that the present three diffirent families. All the
species were described detailedly, with the SEM illustration of spicules and skeleton.
This study is the first approach on the deep-sea sponge in China. The above results
provide important supplements for the deep sea sponge taxonomy study in China.
They can be also served as valuable background for the subsequent sponge taxonomic
The phylogenetic relationships of the 24 species and their related species were
also studied with molecular methods. Partial mitochondrial 16S ribosomal RNA genes
of 21 species were amplified (the three species with dead specimens were not
included in the molecular study). Based on the amplicons, a phylogentic tree was built
and performed preliminary investigation into the relationship of these species. The
primers for sponge molecular research were also experimented, because up to date,
there were no suitable universal primers for all the sponges groups. The existed universal primers were usually corresponding to different taxonomic categories. In
this study, primer 16S1fw and 16SHmod were found efficient for both our
Demospongiae and glass sponge samples. The availability of this pair of primers in
other sponge groups should be test in the subsequent studies.
Subject Area生物学
Document Type学位论文
Recommended Citation
GB/T 7714
龚琳. 西太平洋深海海绵动物分类学及其相关种属的分子系统发育关系初步研究[D]. 北京. 中国科学院大学,2016.
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