| Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica | |
Zhang Wenjing1,2; Sun Delin3; Zhao Xia4 ; Jin Weihua1; Wang Jing1; Zhang Quanbin1
| |
| 2016 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 卷号 | 34期号:1页码:177-185 |
| 文章类型 | Article |
| 摘要 | A rapid, sensitive and reproducible high performance liquid chromatography (HPLC) method with post-column fluorescence derivatization has been developed to determine the amount of low-molecular-weight sulfated polysaccharide (GFS) in vivo. The metabolism of GFS has been shown to fit a two component model following its administration by intravenous injection, and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase (t (1/2 alpha))=11.24 +/- 2.93 min, half-time of elimination phase (t (1/2 beta))=98.20 +/- 25.78 min, maximum concentration (C (max))=110.53 mu g/mL and peak time (T (max))=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However, the concentration of GFS found in serum was too low for detection, and GFS could only be detected for up to 2 h after intragastric administration (200 mg/kg body weight). Thus, the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion, HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection. |
| 关键词 | Sulfated Polysaccharide Hplc Post-column Derivatization Microanalysis Pharmacokinetics |
| DOI | 10.1007/s00343-015-4273-9 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370345300018 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/112475 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Heze Junxinyuan Food Co, Heze 274415, Peoples R China 4.Ocean Univ China, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Wenjing,Sun Delin,Zhao Xia,et al. Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(1):177-185. |
| APA | Zhang Wenjing,Sun Delin,Zhao Xia,Jin Weihua,Wang Jing,&Zhang Quanbin.(2016).Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(1),177-185. |
| MLA | Zhang Wenjing,et al."Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.1(2016):177-185. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Microanalysis and pr（523KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论