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Characteristics of Global Ocean Abnormal Mesoscale Eddies Derived From the Fusion of Sea Surface Height and Temperature Data by Deep Learning 期刊论文
GEOPHYSICAL RESEARCH LETTERS, 2021, 卷号: 48, 期号: 17, 页码: 11
Authors:  Liu, Yingjie;  Zheng, Quanan;  Li, Xiaofeng
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meososcale eddies  abnormal eddies  multi-source remote sensing data  deep learning  data fusion  statistical analysis of spatiotemporal characteristics  
Deep-learning-based information mining from ocean remote-sensing imagery 期刊论文
NATIONAL SCIENCE REVIEW, 2020, 卷号: 7, 期号: 10, 页码: 1584-1605
Authors:  Li, Xiaofeng;  Liu, Bin;  Zheng, Gang;  Ren, Yibin;  Zhang, Shuangshang;  Liu, Yingjie;  Gao, Le;  Liu, Yuhai;  Zhang, Bin;  Wang, Fan
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ocean remote sensing  big data  artificial intelligence  image classification  
Characterization of Sea Surface Temperature and Air-Sea Heat Flux Anomalies Associated With Mesoscale Eddies in the South China Sea 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS, 2020, 卷号: 125, 期号: 4, 页码: 19
Authors:  Liu, Yingjie;  Yu, Lisan;  Chen, Ge
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mesoscale eddies  air-sea coupling  South China Sea  
Structural basis for specific calcium binding by the polycystic-kidney-disease domain of Vibrio anguillarum protease Epp 期刊论文
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 卷号: 505, 期号: 2, 页码: 471-477
Authors:  Li, Peihai;  Zang, Kun;  Li, Yingjie;  Liu, Changshui;  Ma, Qingjun
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Vibrio anguillarum  Polycystic-kidney-disease domain  Protease maturation  Ca2+-binding site  Protein stability  
The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies 期刊论文
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2018, 卷号: 74, 页码: 283-287
Authors:  Han, Yu;  Zang, Kun;  Liu, Changshui;  Li, Yingjie;  Ma, Qingjun
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Vibrio anguillarum  siderophore-interacting proteins  iron release  siderophores  
Structural and enzymatic analysis of the cytochrome b(5) reductase domain of Ulva prolifera nitrate reductase 期刊论文
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 卷号: 111, 页码: 1175-1182
Authors:  You, Cai;  Liu, Changshui;  Li, Yingjie;  Jiang, Peng;  Ma, Qingjun
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Ulva prolifera  Nitrate reductase  Indel  Cytochrome b(5) reductase  
一种制备别藻蓝蛋白三聚体荧光蛋白的方法 专利
专利类型: 发明, 专利号: CN200910255643.X, 申请日期: 2011-06-15, 公开日期: 2011-06-15
Inventors:  刘少芳;  陈英杰;  路延笃;  陈华新;  李富超;  秦松
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一种制备别藻蓝蛋白三聚体荧光蛋白的方法  其特征在于:首先将别藻蓝蛋白α亚基脱辅基蛋白基因与藻胆素生物合成酶基因ho1和pcya克隆到一个表达载体中  其次将别藻蓝蛋白β亚基脱辅基蛋白基因与色基裂合酶基因cpcs和cpcu克隆到另一个载体上  将两个表达载体同时转化大肠杆菌  筛选出同时表达上述基因的工程菌  即为别藻蓝蛋白三聚体荧光蛋白。  
Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles 期刊论文
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 卷号: 879, 期号: 13-14, 页码: 993-997
Authors:  Chen, Yingjie;  Jiang, Peng;  Liu, Shaofang;  Zhao, Hui;  Cui, Yulin;  Qin, Song
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6xhis Tag  Phycobiliprotein  Silica-coated Magnetic Nanoparticles  (Simnps)  Zn(Ii)  Purification  Adsorption Behavior  
一种链酶亲和素结合功能荧光磁性纳米颗粒的制备方法 专利
专利类型: 发明, 专利号: CN201010261766.7, 申请日期: 2011-04-13, 公开日期: 2011-04-13
Inventors:  陈英杰;  秦松;  刘少芳;  崔玉琳;  姜鹏;  陈华新;  李富超
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一种链酶亲和素结合功能荧光磁性纳米颗粒的制备方法  其特征在于:1)通过pcr反应将strep?ii标签基因分别连在别藻蓝蛋白apc的α和β亚基脱辅基蛋白基因的n末端  2)将strep?ii?apca基因片段插入到带有6×his基因的载体a中6×his基因的下游  分别得到strep?ii?apca和strep?ii?apcb基因片段  同时将藻胆素生物合成酶基因hol和pcya插入到载体上  将strep?ii?apcb基因片段插入到带有6×his基因的载体b中6×his基因的下游  待用  得到pcdf?strep?ii?apca?hol?pcya  同时将色基裂合酶基因cpcs和cpcu插入到载体上  3)将步骤2)所得两个表达载体同时转化大肠杆菌  待用  得到prsf?strep?ii?apcb?cpcs?cpcu  筛选获得同时表达上述基因的工程菌  4)将上述工程菌诱导培养后分离纯化  待用  得到双标签重组apc荧光蛋白质  5)将所得双标签重组apc荧光蛋白质与锌离子修饰的超顺磁性硅壳纳米颗粒振荡混合  即得到链酶亲和素结合功能荧光磁性纳米颗粒。  
抗氧化活性藻蓝色素与牛血清白蛋白的相互作用 期刊论文
药物分析杂志, 2011, 期号: 1, 页码: 87-89
Authors:  陈英杰;  刘少芳;  陈华新;  李富超;  秦松
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藻蓝色素(Pcb)  牛血清白蛋白(Bsa)  荧光淬灭