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进行凡纳滨对虾家系鉴定的多重微卫星鉴定体系及应用 专利
专利类型: 发明, 专利号: CN201310340730.1, 申请日期: 2013-12-25, 公开日期: 2013-12-25
发明人:  于洋;  李富花;  张晓军;  相建海
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进行凡纳滨对虾家系鉴定的多重微卫星鉴定体系  包括多重微卫星引物体系  其特征在于:使用多态性高的lvm1‑lvm15共15对微卫星引物构成两个多重微卫星引物体系  所述的两个多重微卫星引物体系如下:1)多重微卫星引物体系1的引物序列和荧光标记组合为:lvm1f:6‑fam(羧基荧光素)‑5’‑gatgacctgcctttctctgc‑3’lvm1r:5’‑gggagagatgatgggaagaag‑3’lvm2f:Rox(羧基x罗丹明)‑5’‑atggcacaaataggatcttg‑3’lvm2r:5’‑gactggaagagcactgattc‑3’lvm3f:Hex(六氯荧光素)‑5’‑atacgaaacaccccatccc‑3’lvm3r:5’‑gtggtcttacctcgtggctc‑3’lvm4f:Tamra(羧基四甲基罗丹明)‑5’‑agcacctagcacttgctgaac‑3’lvm4r:5’‑agagactcacatcctcatcctc‑3’lvm5f:6‑fam(羧基荧光素)‑5’‑cattgaaaacggaatcctcg‑3’lvm5r:5’‑gatattcccatcaacacagcg‑3’lvm6f:Rox(羧基x罗丹明)‑5’‑caccaaaacgaacgaaacg‑3’lvm6r:5’‑ggataaaaacgaattgtataccg‑3’lvm7f:Hex(六氯荧光素)‑5’‑gagagcaaataagaaagggc‑3’lvm7r:5’‑aggatgcaaatgataacgag‑3’lvm8f:Tamra(羧基四甲基罗丹明)‑5’‑ggcacactgtttagtcctcg‑3’lvm8r:5’‑cgaacagaatggcagaggag‑3’lvm9f:6‑fam(羧基荧光素)‑5’‑cccgacttggcttttagttg‑3’lvm9r:5’‑gagattgctatcctcggctg‑3’lvm10f:Rox(羧基x罗丹明)‑5’‑gatcctgcgagtcactttatctc‑3’lvm10r:5’‑tttattgcgtatcccagaagc‑3’2)多重微卫星引物体系2的引物和荧光标记组合如下:lvm11f:6‑fam(羧基荧光素)‑5’‑ccgccatcatcatcaaca‑3’lvm11r:5’‑tcattcgggttcgagactc‑3’lvm12f:Rox(羧基x罗丹明)‑5’‑gatcattcgcccctcttttt‑3’lvm12r:5’‑atctacggttcgagagcaga‑3’lvm13f:Hex(六氯荧光素)‑5’‑ggactcacacttctggttc‑3’lvm13r:5’‑ggctgcaccttgtaagtc‑3’lvm14f:Tamra(羧基四甲基罗丹明)‑5’‑tatgctcgttccctttgctt‑3’lvm14r:5’‑ttgaaggaaaagtgttgggg‑3’lvm15f:6‑fam(羧基荧光素)‑5’‑actagtggatctgtctattcat‑3’lvm15r:5’‑atacccacccatgcatgtta‑3’。  
Molecular Cloning, Characterization, and Expression of Two Myeloid Differentiation Factor 88 (Myd88) in Pacific Oyster, Crassostrea gigas 期刊论文
JOURNAL OF THE WORLD AQUACULTURE SOCIETY, 2013, 卷号: 44, 期号: 6, 页码: 759-774
作者:  Du, Yishuai;  Zhang, Linlin;  Huang, Baoyu;  Guan, Xudong;  Li, Li;  Zhang, Guofan;  Li, L
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Distribution of large-scale detachment faults on mid-ocean ridges in relation to spreading rates 期刊论文
ACTA OCEANOLOGICA SINICA, 2013, 卷号: 32, 期号: 12, 页码: 109-117
作者:  Yu Zhiteng;  Li Jiabiao;  Liang Yuyang;  Han Xiqiu;  Zhang Jie;  Zhu Lei;  Li, JB
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Detachment Faults  Oceanic Core Complex  Spreading Rates  Distribution  Tectonism  Magma Supply  Hydrothermal Vents  
Roles of mixotrophy in blooms of different dinoflagellates: Implications from the growth experiment 期刊论文
HARMFUL ALGAE, 2013, 卷号: 30, 页码: 10-26
作者:  Zhang, Qing-Chun;  Song, Jing-Jing;  Yu, Ren-Cheng;  Yan, Tian;  Wang, Yun-Feng;  Kong, Fan-Zhou;  Zhou, Ming-Jiang;  Yu, RC
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Harmful Algal Bloom  Mixotrophy  Karenia Mikimotoi  Alexandrium Catenella  Prorocentrum Micans  Prorocentrum Donghaiense  
中国蛇绿岩型铬铁矿的研究总结及思考 期刊论文
矿物学报, 2013, 期号: S2, 页码: 95
作者:  胡振兴;  牛耀龄;  刘益;  张国瑞;  孙文礼;  马玉鑫
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蛇绿岩:6944  豆荚状铬铁矿:5598  大道尔吉:1969  内蒙古:1805  西藏罗布莎:1660  岩反应:1568  造山带:1499  古亚洲洋:1419  思考:1392  分布不均衡:1370  
Development of a triplex loop-mediated isothermal amplification method for rapid on-site detection of three Vibrio species associated with fish diseases 期刊论文
AQUACULTURE, 2013, 卷号: 414, 页码: 267-273
作者:  Yu, Lan-ping;  Hu, Yong-hua;  Zhang, Xiao-hua;  Sun, Bo-guang;  Sun, BG
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Loop-mediated Isothermal Amplification  Vibrio Harveyi  Vibrio Anguillarum  Vibrio Alginolyticus  On-site Detection  
The role of nonlinearities associated with air-sea coupling processes in El Nino's peak-phase locking 期刊论文
SCIENCE CHINA-EARTH SCIENCES, 2013, 卷号: 56, 期号: 11, 页码: 1988-1996
作者:  Duan WanSuo;  Zhang Rui;  Yu YanShan;  Tian Ben;  Duan, WS
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El Nino Event  Nonlinearity  Optimal Perturbation  Numerical Model  
Tracing the origin of paralytic shellfish toxins in scallop Patinopecten yessoensis in the northern Yellow Sea 期刊论文
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2013, 卷号: 30, 期号: 11, 页码: 1933-1945
作者:  Chen, Jian-Hua;  Yu, Ren-Cheng;  Gao, Yan;  Kong, Fan-Zhou;  Wang, Yun-Feng;  Zhang, Qing-Chun;  Kang, Zhen-Jun;  Yan, Tian;  Zhou, Ming-Jiang;  Yu, RC
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Paralytic Shellfish Toxins (Pst)  Alexandrium  Patinopecten Yessoensis  Yellow Sea  Hplc  Single-cell Pcr  
The involvement of PDGF/VEGF related factor in regulation of immune and neuroendocrine in Chinese mitten crab Eriocheir sinensis 期刊论文
FISH & SHELLFISH IMMUNOLOGY, 2013, 卷号: 35, 期号: 4, 页码: 1240-1248
作者:  Li, Fengmei;  Xu, Li;  Gai, Xuemei;  Zhou, Zhi;  Wang, Lingling;  Zhang, Huan;  Gai, Yunchao;  Song, Linsheng;  Yu, Jiansheng;  Liang, Chengwei;  Li, FM
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Pvf1  Eriocheir Sinensis  Immune Response  Neuroendocrine  
Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment 期刊论文
JOURNAL OF INVERTEBRATE PATHOLOGY, 2013, 卷号: 114, 期号: 2, 页码: 144-150
作者:  Liu, Rui;  Qiu, Limei;  Yu, Zuoan;  Zi, Jun;  Yue, Feng;  Wang, Lingling;  Zhang, Huan;  Teng, Weiming;  Liu, Xiangfeng;  Song, Linsheng;  Song, LS
Adobe PDF(1564Kb)  |  收藏  |  浏览/下载:310/0  |  提交时间:2014/07/17
Pathogencity  Bacterial Infection  Bivalve Disease  Lesion  Low Temperature