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Biosynthesis of a Phycocyanin Beta Subunit with Two Noncognate Chromophores in Escherichia coli 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2020, 卷号: 191, 期号: 2, 页码: 763-771
作者:  Chen, Huaxin;  Zheng, Caiyun;  Jiang, Peng;  Ji, Gengsheng
Adobe PDF(536Kb)  |  收藏  |  浏览/下载:115/0  |  提交时间:2020/09/25
Phycobiliprotein  Phycocyanin  Lyase  Fluorescence  Chromophore  E  coli  
Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein 期刊论文
MICROBIAL CELL FACTORIES, 2019, 卷号: 18, 页码: 13
作者:  Chen, Huaxin;  Jiang, Peng
Adobe PDF(1664Kb)  |  收藏  |  浏览/下载:176/0  |  提交时间:2019/05/15
Phycobiliprotein  Phycobilin  Chromophorylation ratio  Plasmid stability  E. coli  
Combinational biosynthesis and characterization of fusion proteins with tandem repeats of allophycocyanin holo-alpha subunits, and their application as bright fluorescent labels for immunofluorescence assay 期刊论文
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2018, 卷号: 126, 期号: 6, 页码: 778-782
作者:  Chen, Huaxin;  Jiang, Peng
Adobe PDF(931Kb)  |  收藏  |  浏览/下载:308/0  |  提交时间:2019/05/15
Allophycocyanin  Streptavidin  Fusion protein  Fluorescence  Immunofluorescence assay  
Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit 期刊论文
BIOENGINEERED, 2018, 卷号: 9, 期号: 1, 页码: 108-115
作者:  Wu, Jing;  Chen, Huaxin;  Jiang, Peng
Adobe PDF(997Kb)  |  收藏  |  浏览/下载:288/0  |  提交时间:2019/08/21
Allophycocyanin  Chromophore attachment  Chromophorylation rate  Immunofluorescence assay  Phycobiliprotein  
Fusion proteins of streptavidin and allophycocyanin alpha subunit for immunofluorescence assay 期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2017, 卷号: 125, 页码: 97-103
作者:  Wu, Jing;  Chen, Huaxin;  Zhao, Jin;  Jiang, Peng
Adobe PDF(1237Kb)  |  收藏  |  浏览/下载:239/0  |  提交时间:2018/01/11
Streptavidin  Fusion Protein  Biotin  Immunofluorescence Assay  
Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay 期刊论文
PROCESS BIOCHEMISTRY, 2017, 卷号: 58, 页码: 306-312
作者:  Ge, Baosheng;  Lin, Xiaojun;  Chen, Yao;  Wang, Xiangfa;  Chen, Huaxin;  Jiang, Peng;  Huang, Fang
Adobe PDF(615Kb)  |  收藏  |  浏览/下载:221/0  |  提交时间:2017/09/28
Combinational Biosynthesis  Streptavidin-phycobiliproteins  Immunoassay  Biomarkers  
Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome 期刊论文
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2015, 卷号: 33, 期号: 4, 页码: 819-827
作者:  Zhang, Hao;  Li, Fuchao;  Chen, Huaxin;  Zhao, Jin;  Yan, Jinfei;  Jiang, Peng;  Li, Ronggui;  Zhu, Baoli
Adobe PDF(723Kb)  |  收藏  |  浏览/下载:290/1  |  提交时间:2015/07/27
Marine Sediment  Metagenome  Lipolytic  Expression  Esterase Characterization  
Biosynthesis of a stable allophycocyanin beta subunit in metabolically engineered Escherichia coli 期刊论文
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2013, 卷号: 115, 期号: 5, 页码: 485-489
作者:  Chen, Huaxin;  Lin, Hanzhi;  Li, Fuchao;  Jiang, Peng;  Qin, Song;  Jiang, P
Adobe PDF(539Kb)  |  收藏  |  浏览/下载:275/0  |  提交时间:2014/07/17
Allophycocyanin  Biosynthesis  Escherichia Coli  Fluorescence  Thermostability  
Biosynthesis of fluorescent cyanobacterial allophycocyanin trimer in Escherichia coli 期刊论文
PHOTOSYNTHESIS RESEARCH, 2010, 卷号: 105, 期号: 2, 页码: 135-142
作者:  Liu, Shaofang;  Chen, Yingjie;  Lu, Yandu;  Chen, Huaxin;  Li, Fuchao;  Qin, Song
Adobe PDF(311Kb)  |  收藏  |  浏览/下载:211/0  |  提交时间:2010/12/24
Allophycocyanin  Trimer Assembly  Recombinant Protein  Allophycocyanin Monomer  Fluorescence  Escherichia Coli  
Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli 期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2009, 卷号: 48, 期号: 1, 页码: 58-64
作者:  Liu, Shaofang;  Chen, Huaxin;  Qin, Song;  Zhang, Weijie;  Guan, Xiangyu;  Lu, Yandu
Adobe PDF(681Kb)  |  收藏  |  浏览/下载:356/1  |  提交时间:2010/12/22
Maltose-binding Protein  Phycocyanin-alpha Subunit  Protein Engineering  Recombinant Protein  Chromatography  Recombinant Protein Production