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Biosynthesis of a Phycocyanin Beta Subunit with Two Noncognate Chromophores in Escherichia coli 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2020, 卷号: 191, 期号: 2, 页码: 763-771
作者:  Chen, Huaxin;  Zheng, Caiyun;  Jiang, Peng;  Ji, Gengsheng
Adobe PDF(536Kb)  |  收藏  |  浏览/下载:119/0  |  提交时间:2020/09/25
Phycobiliprotein  Phycocyanin  Lyase  Fluorescence  Chromophore  E  coli  
Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein 期刊论文
MICROBIAL CELL FACTORIES, 2019, 卷号: 18, 页码: 13
作者:  Chen, Huaxin;  Jiang, Peng
Adobe PDF(1664Kb)  |  收藏  |  浏览/下载:177/0  |  提交时间:2019/05/15
Phycobiliprotein  Phycobilin  Chromophorylation ratio  Plasmid stability  E. coli  
Combinational biosynthesis and characterization of fusion proteins with tandem repeats of allophycocyanin holo-alpha subunits, and their application as bright fluorescent labels for immunofluorescence assay 期刊论文
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2018, 卷号: 126, 期号: 6, 页码: 778-782
作者:  Chen, Huaxin;  Jiang, Peng
Adobe PDF(931Kb)  |  收藏  |  浏览/下载:309/0  |  提交时间:2019/05/15
Allophycocyanin  Streptavidin  Fusion protein  Fluorescence  Immunofluorescence assay  
Structural and enzymatic analysis of the cytochrome b(5) reductase domain of Ulva prolifera nitrate reductase 期刊论文
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 卷号: 111, 页码: 1175-1182
作者:  You, Cai;  Liu, Changshui;  Li, Yingjie;  Jiang, Peng;  Ma, Qingjun
Adobe PDF(3475Kb)  |  收藏  |  浏览/下载:263/0  |  提交时间:2019/08/21
Ulva prolifera  Nitrate reductase  Indel  Cytochrome b(5) reductase  
Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit 期刊论文
BIOENGINEERED, 2018, 卷号: 9, 期号: 1, 页码: 108-115
作者:  Wu, Jing;  Chen, Huaxin;  Jiang, Peng
Adobe PDF(997Kb)  |  收藏  |  浏览/下载:297/0  |  提交时间:2019/08/21
Allophycocyanin  Chromophore attachment  Chromophorylation rate  Immunofluorescence assay  Phycobiliprotein  
Cloning and characterization of nitrate reductase gene in Ulva prolifera (Ulvophyceae, Chlorophyta) 期刊论文
JOURNAL OF PHYCOLOGY, 2017, 卷号: 53, 期号: 5, 页码: 1035-1043
作者:  Guo, Yang;  Wang, Hao Zhe;  Wu, Chun Hui;  Fu, Hui Hui;  Jiang, Peng
Adobe PDF(1118Kb)  |  收藏  |  浏览/下载:230/0  |  提交时间:2018/01/11
Ammonium  Expression Analysis  Gene Cloning  Green Tide  Nitrate  Nitrate Reductase  Ulva Prolifera  
Fusion proteins of streptavidin and allophycocyanin alpha subunit for immunofluorescence assay 期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2017, 卷号: 125, 页码: 97-103
作者:  Wu, Jing;  Chen, Huaxin;  Zhao, Jin;  Jiang, Peng
Adobe PDF(1237Kb)  |  收藏  |  浏览/下载:239/0  |  提交时间:2018/01/11
Streptavidin  Fusion Protein  Biotin  Immunofluorescence Assay  
Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay 期刊论文
PROCESS BIOCHEMISTRY, 2017, 卷号: 58, 页码: 306-312
作者:  Ge, Baosheng;  Lin, Xiaojun;  Chen, Yao;  Wang, Xiangfa;  Chen, Huaxin;  Jiang, Peng;  Huang, Fang
Adobe PDF(615Kb)  |  收藏  |  浏览/下载:221/0  |  提交时间:2017/09/28
Combinational Biosynthesis  Streptavidin-phycobiliproteins  Immunoassay  Biomarkers  
Biosynthesis, spectral properties and thermostability of cyanobacterial allophycocyanin holo-alpha subunits 期刊论文
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2016, 卷号: 88, 页码: 88-92
作者:  Chen, Huaxin;  Liu, Qiuzi;  Zhao, Jin;  Jiang, Peng
Adobe PDF(626Kb)  |  收藏  |  浏览/下载:218/0  |  提交时间:2016/08/24
Allophycocyanin  Escherichia Coli  Thermostability  Fluorescence  
Biosynthesis of a stable allophycocyanin beta subunit in metabolically engineered Escherichia coli 期刊论文
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2013, 卷号: 115, 期号: 5, 页码: 485-489
作者:  Chen, Huaxin;  Lin, Hanzhi;  Li, Fuchao;  Jiang, Peng;  Qin, Song;  Jiang, P
Adobe PDF(539Kb)  |  收藏  |  浏览/下载:282/0  |  提交时间:2014/07/17
Allophycocyanin  Biosynthesis  Escherichia Coli  Fluorescence  Thermostability