IOCAS-IR

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Biosynthesis of a Phycocyanin Beta Subunit with Two Noncognate Chromophores in Escherichia coli 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2020, 卷号: 191, 期号: 2, 页码: 763-771
作者:  Chen, Huaxin;  Zheng, Caiyun;  Jiang, Peng;  Ji, Gengsheng
Adobe PDF(536Kb)  |  收藏  |  浏览/下载:119/0  |  提交时间:2020/09/25
Phycobiliprotein  Phycocyanin  Lyase  Fluorescence  Chromophore  E  coli  
Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein 期刊论文
MICROBIAL CELL FACTORIES, 2019, 卷号: 18, 页码: 13
作者:  Chen, Huaxin;  Jiang, Peng
Adobe PDF(1664Kb)  |  收藏  |  浏览/下载:177/0  |  提交时间:2019/05/15
Phycobiliprotein  Phycobilin  Chromophorylation ratio  Plasmid stability  E. coli  
Combinational biosynthesis and characterization of fusion proteins with tandem repeats of allophycocyanin holo-alpha subunits, and their application as bright fluorescent labels for immunofluorescence assay 期刊论文
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2018, 卷号: 126, 期号: 6, 页码: 778-782
作者:  Chen, Huaxin;  Jiang, Peng
Adobe PDF(931Kb)  |  收藏  |  浏览/下载:309/0  |  提交时间:2019/05/15
Allophycocyanin  Streptavidin  Fusion protein  Fluorescence  Immunofluorescence assay  
Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit 期刊论文
BIOENGINEERED, 2018, 卷号: 9, 期号: 1, 页码: 108-115
作者:  Wu, Jing;  Chen, Huaxin;  Jiang, Peng
Adobe PDF(997Kb)  |  收藏  |  浏览/下载:297/0  |  提交时间:2019/08/21
Allophycocyanin  Chromophore attachment  Chromophorylation rate  Immunofluorescence assay  Phycobiliprotein  
Fusion proteins of streptavidin and allophycocyanin alpha subunit for immunofluorescence assay 期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2017, 卷号: 125, 页码: 97-103
作者:  Wu, Jing;  Chen, Huaxin;  Zhao, Jin;  Jiang, Peng
Adobe PDF(1237Kb)  |  收藏  |  浏览/下载:239/0  |  提交时间:2018/01/11
Streptavidin  Fusion Protein  Biotin  Immunofluorescence Assay  
藻胆蛋白荧光探针的生物合成及其在免疫荧光检测中的应用研究 学位论文
, 北京: 中国科学院大学, 2017
作者:  武静
Adobe PDF(4017Kb)  |  收藏  |  浏览/下载:255/1  |  提交时间:2017/06/12
Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli 期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2009, 卷号: 48, 期号: 1, 页码: 58-64
作者:  Liu, Shaofang;  Chen, Huaxin;  Qin, Song;  Zhang, Weijie;  Guan, Xiangyu;  Lu, Yandu
Adobe PDF(681Kb)  |  收藏  |  浏览/下载:356/1  |  提交时间:2010/12/22
Maltose-binding Protein  Phycocyanin-alpha Subunit  Protein Engineering  Recombinant Protein  Chromatography  Recombinant Protein Production