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A novel junctional adhesion molecule A (CgJAM-A-L) from oyster (Crassostrea gigas) functions as pattern recognition receptor and opsonin
Liu, Conghui1,3; Wang, Mengqiang1; Jiang, Shuai1; Wang, Lingling1; Chen, Hao1,3; Liu, Zhaoqun1,3; Qiu, Limei1; Song, Linsheng2
2016-02-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume55Issue:2016Pages:211-220
SubtypeArticle
AbstractJunctional adhesion molecule (JAM), a subfamily of immunoglobulin superfamily (IgSF) with a couple of immunoglobulin domains, can act as regulator in homeostasis and inflammation of vertebrates. In the present study, a structural homolog of JAM-A (designated CgJAM-A-L) was screened out from oyster, Crassostrea gigas, through a search of JAM-A D1 domain (N-terminal Ig domain in JAM-A). The cDNA of CgJAM-A-L was of 1188 bp encoding a predicted polypeptide of 395 amino acids. The immunoreactive area of CgJAM-A-L mainly distributed over the plasma membrane of hemocytes. After Vi bra splendidus or tumor necrosis factor (CgTNE-1) stimulation, the mRNA transcripts of CgJAM-A-L in hemocytes increased significantly by 4.46-fold and 9.00-fold (p <0.01) of those in control group, respectively. The recombinant CgJAM-A-L protein (rCgJAM-A-L) could bind multiple PAMPs including lipopolysaccharides (LPS), peptidoglycan (PGN), lipoteichoic acid (LTA), mannose (MAN), beta-glucan (GLU) and poly(I:C), and various microorganisms including Micrococcus luteus, Staphylococcus aureus, Escherichia coli, Vibro anguillarum, V. splendidus, Pastoris pastoris and Yarrowia lipolytica. The phagocytic rates of oyster hemocytes towards Gram-negative bacteria V. anguillarum and yeast P. pastoris were significantly enhanced after the incubation of rCgJAM-A-L, and even increased more significantly after the pre-incubation of rCgJAM-A-L with microbes (p <0.01). The results collectively indicated that CgJAM-A-L functioned as an important pattern recognition receptor (PRR) and opsonin in the immune defense against invading pathogen in oyster. Moreover, as the most primitive specie with homolog of JAMs, the information of CgJAM-A-L in oyster would provide useful clues for the evolutionary study of JAMs and immunoglobulins. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordJunctional Adhesion Molecule Immunoglobulin Superfamily Innate Immune Response Prr Opsonin Evolution Pattern
DOI10.1016/j.dci.2015.09.011
Indexed BySCI
Language英语
WOS IDWOS:000368382200024
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/95219
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Conghui,Wang, Mengqiang,Jiang, Shuai,et al. A novel junctional adhesion molecule A (CgJAM-A-L) from oyster (Crassostrea gigas) functions as pattern recognition receptor and opsonin[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,55(2016):211-220.
APA Liu, Conghui.,Wang, Mengqiang.,Jiang, Shuai.,Wang, Lingling.,Chen, Hao.,...&Song, Linsheng.(2016).A novel junctional adhesion molecule A (CgJAM-A-L) from oyster (Crassostrea gigas) functions as pattern recognition receptor and opsonin.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,55(2016),211-220.
MLA Liu, Conghui,et al."A novel junctional adhesion molecule A (CgJAM-A-L) from oyster (Crassostrea gigas) functions as pattern recognition receptor and opsonin".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 55.2016(2016):211-220.
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