A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas

doi:10.1016/j.dci.2015.10.025

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	A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas
	Li, Yiqun 1,3; Jiang, Shuai 1; Li, Meijia1,3 ; Xin, Lusheng1,3 ; Wang, Lingling 1; Wang, Hao 1; Qiu, Limei 1; Song, Linsheng 2
	2016-02-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	55 期号:2016 页码:179-187
文章类型	Article
摘要	Astakine has been reported to be a hematopoietic growth factor of prokineticin homolog firstly found in arthropods freshwater crayfish Pacifastacus leniusculus. In the present study, an astakine homologous gene was identified from Pacific oyster Crassostrea gigas (designated CgAstakine). The full length cDNA of CgAstakine encoded a polypeptide of 103 amino acids containing a prokineticin (PK) domain homologous to that in astakine from freshwater crayfish P. leniusculus. The deduced amino acid sequence of CgAstakine shared higher similarity with those of other invertebrate astakines than prokineticins from vertebrates. The mRNA of CgAstakine was highly expressed in hepatopancreas and adductor muscle of oyster, while the CgAstakine protein was mainly distributed in hepatopancreas, gill and hemocytes. The mRNA expression of CgAstaldne in hemocytes was significantly increased (p < 0.01) and maintained at a high level from 3 h to 9 h after Vibrio anguillarum challenge. After the oyster hemocytes were incubated with 5 mu g/mL recombinant CgAstakine protein (rCgAstakine) for 24 h in vitro, the proliferation of hemocytes was significantly increased to 1.89 fold of that in control group (p < 0.05). Moreover, the total count of oyster hemocytes was significantly upregulated (2.45 fold of that in control group, p < 0.05) at 12 h after the oysters were received an injection of rCgAstakine (0.5 mu g/g). These results collectively indicated that CgAstakine could modulate the hemocytes proliferation both in vitro and in vivo, and probably involved in the hematopoietic process fighting against the invasion of foreign pathogens. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Crassostrea Gigas Hematopoiesis Innate Immunity Astakine Hemocyte Proliferation
DOI	10.1016/j.dci.2015.10.025
收录类别	SCI
语种	英语
WOS记录号	WOS:000368382200020
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/95217
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Li, Yiqun,Jiang, Shuai,Li, Meijia,et al. A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,55(2016):179-187.
APA	Li, Yiqun.,Jiang, Shuai.,Li, Meijia.,Xin, Lusheng.,Wang, Lingling.,...&Song, Linsheng.(2016).A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,55(2016),179-187.
MLA	Li, Yiqun,et al."A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 55.2016(2016):179-187.

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