IOCAS-IR  > 实验海洋生物学重点实验室
A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas
Li, Yiqun1,3; Jiang, Shuai1; Li, Meijia1,3; Xin, Lusheng1,3; Wang, Lingling1; Wang, Hao1; Qiu, Limei1; Song, Linsheng2
2016-02-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号55期号:2016页码:179-187
文章类型Article
摘要Astakine has been reported to be a hematopoietic growth factor of prokineticin homolog firstly found in arthropods freshwater crayfish Pacifastacus leniusculus. In the present study, an astakine homologous gene was identified from Pacific oyster Crassostrea gigas (designated CgAstakine). The full length cDNA of CgAstakine encoded a polypeptide of 103 amino acids containing a prokineticin (PK) domain homologous to that in astakine from freshwater crayfish P. leniusculus. The deduced amino acid sequence of CgAstakine shared higher similarity with those of other invertebrate astakines than prokineticins from vertebrates. The mRNA of CgAstakine was highly expressed in hepatopancreas and adductor muscle of oyster, while the CgAstakine protein was mainly distributed in hepatopancreas, gill and hemocytes. The mRNA expression of CgAstaldne in hemocytes was significantly increased (p < 0.01) and maintained at a high level from 3 h to 9 h after Vibrio anguillarum challenge. After the oyster hemocytes were incubated with 5 mu g/mL recombinant CgAstakine protein (rCgAstakine) for 24 h in vitro, the proliferation of hemocytes was significantly increased to 1.89 fold of that in control group (p < 0.05). Moreover, the total count of oyster hemocytes was significantly upregulated (2.45 fold of that in control group, p < 0.05) at 12 h after the oysters were received an injection of rCgAstakine (0.5 mu g/g). These results collectively indicated that CgAstakine could modulate the hemocytes proliferation both in vitro and in vivo, and probably involved in the hematopoietic process fighting against the invasion of foreign pathogens. (C) 2015 Elsevier Ltd. All rights reserved.
关键词Crassostrea Gigas Hematopoiesis Innate Immunity Astakine Hemocyte Proliferation
DOI10.1016/j.dci.2015.10.025
收录类别SCI
语种英语
WOS记录号WOS:000368382200020
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/95217
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Li, Yiqun,Jiang, Shuai,Li, Meijia,et al. A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,55(2016):179-187.
APA Li, Yiqun.,Jiang, Shuai.,Li, Meijia.,Xin, Lusheng.,Wang, Lingling.,...&Song, Linsheng.(2016).A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,55(2016),179-187.
MLA Li, Yiqun,et al."A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 55.2016(2016):179-187.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
A cytokine-like fact(2301KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Yiqun]的文章
[Jiang, Shuai]的文章
[Li, Meijia]的文章
百度学术
百度学术中相似的文章
[Li, Yiqun]的文章
[Jiang, Shuai]的文章
[Li, Meijia]的文章
必应学术
必应学术中相似的文章
[Li, Yiqun]的文章
[Jiang, Shuai]的文章
[Li, Meijia]的文章
相关权益政策
暂无数据
收藏/分享
文件名: A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。