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A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas
Li, Yiqun1,3; Jiang, Shuai1; Li, Meijia1,3; Xin, Lusheng1,3; Wang, Lingling1; Wang, Hao1; Qiu, Limei1; Song, Linsheng2
2016-02-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume55Issue:2016Pages:179-187
SubtypeArticle
AbstractAstakine has been reported to be a hematopoietic growth factor of prokineticin homolog firstly found in arthropods freshwater crayfish Pacifastacus leniusculus. In the present study, an astakine homologous gene was identified from Pacific oyster Crassostrea gigas (designated CgAstakine). The full length cDNA of CgAstakine encoded a polypeptide of 103 amino acids containing a prokineticin (PK) domain homologous to that in astakine from freshwater crayfish P. leniusculus. The deduced amino acid sequence of CgAstakine shared higher similarity with those of other invertebrate astakines than prokineticins from vertebrates. The mRNA of CgAstakine was highly expressed in hepatopancreas and adductor muscle of oyster, while the CgAstakine protein was mainly distributed in hepatopancreas, gill and hemocytes. The mRNA expression of CgAstaldne in hemocytes was significantly increased (p < 0.01) and maintained at a high level from 3 h to 9 h after Vibrio anguillarum challenge. After the oyster hemocytes were incubated with 5 mu g/mL recombinant CgAstakine protein (rCgAstakine) for 24 h in vitro, the proliferation of hemocytes was significantly increased to 1.89 fold of that in control group (p < 0.05). Moreover, the total count of oyster hemocytes was significantly upregulated (2.45 fold of that in control group, p < 0.05) at 12 h after the oysters were received an injection of rCgAstakine (0.5 mu g/g). These results collectively indicated that CgAstakine could modulate the hemocytes proliferation both in vitro and in vivo, and probably involved in the hematopoietic process fighting against the invasion of foreign pathogens. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Hematopoiesis Innate Immunity Astakine Hemocyte Proliferation
DOI10.1016/j.dci.2015.10.025
Indexed BySCI
Language英语
WOS IDWOS:000368382200020
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/95217
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Yiqun,Jiang, Shuai,Li, Meijia,et al. A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,55(2016):179-187.
APA Li, Yiqun.,Jiang, Shuai.,Li, Meijia.,Xin, Lusheng.,Wang, Lingling.,...&Song, Linsheng.(2016).A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,55(2016),179-187.
MLA Li, Yiqun,et al."A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 55.2016(2016):179-187.
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