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Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus
Wen, Aiyun1,2; You, Feng1; Sun, Peng1; Li, Jun1; Xu, Dongdong1; Wu, Zhihao1; Ma, Deyou1; Zou, Yuxia1; Tan, Xungang1; Fan, Zhaofei1; Zhang, Peijun1
2015
Source PublicationANIMAL BIOLOGY
Volume65Issue:3-4Pages:193-207
SubtypeArticle
AbstractThe present study aims to elucidate the different expression patterns and possible roles of Doublesex and Mab-3-related transcription factor 1 (dmrt1), dmrt4, SRY-related transcription factor 9 (sox9) and cytochrome P450 aromatase 19a (cyp19a) during gonadal differentiation in olive flounder, Paralichthys olivaceus. We first analyzed the gene expression patterns in tissues using RT-PCR, which indicated dmrt1, sox9 and cyp19a were sex-related genes with sexual dimorphic expression. The quantitative expression changes of these three genes together with dmrt4 during gonadal differentiation were further examined using real-time RT-PCR. The results showed that dmrt1 was scarcely expressed in the primitive gonad and during following periods of gonadal differentiation. Its expression increased rapidly in the differentiating testis. Dmrt4 was strongly expressed in primitive gonads and much less expressed during following periods of gonadal differentiation. Its expression became strong in differentiating testes. While sox9 was highly expressed in the primitive gonad, it was expressed with fluctuations during following periods of gonadal differentiation. Cyp19a started expressing in primitive gonads, and its expression quantity fluctuated during latter periods of gonadal differentiation, but was strongly expressed in the early stage of differentiating ovaries. Results of in situ hybridization showed that dmrt4 and sox9 transcripts were both mainly localized in spermatocytes and our results suggested these four sex-related genes might be involved in gonadal differentiation through their synergistic effects in flounder.
KeywordExpression Patterns Gonadal Differentiation Paralichthys Olivaceus Sex Related Genes
DOI10.1163/15707563-00002470
Indexed BySCI
Language英语
WOS IDWOS:000367519100001
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/95208
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wen, Aiyun,You, Feng,Sun, Peng,et al. Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus[J]. ANIMAL BIOLOGY,2015,65(3-4):193-207.
APA Wen, Aiyun.,You, Feng.,Sun, Peng.,Li, Jun.,Xu, Dongdong.,...&Zhang, Peijun.(2015).Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus.ANIMAL BIOLOGY,65(3-4),193-207.
MLA Wen, Aiyun,et al."Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus".ANIMAL BIOLOGY 65.3-4(2015):193-207.
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