IOCAS-IR  > 海洋生态与环境科学重点实验室
Effects of nitrogenous nutrition on growth and nitrogen assimilation enzymes of dinoflagellate Akashiwo sanguinea
Liu, Yun1,2; Chen, Tiantian1,2,3; Song, Shuqun1,2; Li, Caiwen1,2
2015-12-01
Source PublicationHARMFUL ALGAE
Volume50Issue:2015Pages:99-106
SubtypeArticle
AbstractThe dinoflagellate Akashiwo sanguinea is a cosmopolitan species which frequently causes dense blooms in estuaries and coasts around the world, while the mechanism regulating the formation of the algal bloom remains unclear. To further explore the physiological response of A. sartguinea during algal blooms, we studied the effects of various nitrogenous nutrients on growth and nitrogen assimilation enzymes of A. sanguinea in laboratory trials. Our results indicated that A. sanguinea was able to grow well on multiple N substrates within appropriate ranges of concentrations. The dinoflagellate grew faster in NH4+, while it showed greatest affinity for urea. The intracellular enzymes of A. sanguinea were regulated to adapt for the environmental nutrient conditions and growth stages: nitrate reductase. (NR) was induced when grown on NO while inhibited by NH4+ and urea, glutamine synthetase (GS) expressed constitutively in all N sources, and urease was up-regulated by NO3- and urea but down-regulated by NH4+. Meanwhile, the activities of the N assimilation enzymes decreased with the growth of A. sanguinea, and GS activity showed a significant positive correlation with growth rates. The urease activity of A. sanguinea was extremely high assessed by the kinetics of N assimilation, indicating its competitive advantage for urea over other phytoplankton species. The physiological activity of N assimilation enzymes in A. sanguinea facilitates its utilization of reduced N, and implies its capability of forming blooms in environments where reduced N is the common N source. (C) 2015 Elsevier B.V. All rights reserved.
KeywordDinoflagellate Nitrogen Growth Enzyme Kinetics Algal Blooms
DOI10.1016/j.hal.2015.10.005
Indexed BySCI
Language英语
WOS IDWOS:000367275600010
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/86657
Collection海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qjngdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 10039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Yun,Chen, Tiantian,Song, Shuqun,et al. Effects of nitrogenous nutrition on growth and nitrogen assimilation enzymes of dinoflagellate Akashiwo sanguinea[J]. HARMFUL ALGAE,2015,50(2015):99-106.
APA Liu, Yun,Chen, Tiantian,Song, Shuqun,&Li, Caiwen.(2015).Effects of nitrogenous nutrition on growth and nitrogen assimilation enzymes of dinoflagellate Akashiwo sanguinea.HARMFUL ALGAE,50(2015),99-106.
MLA Liu, Yun,et al."Effects of nitrogenous nutrition on growth and nitrogen assimilation enzymes of dinoflagellate Akashiwo sanguinea".HARMFUL ALGAE 50.2015(2015):99-106.
Files in This Item:
File Name/Size DocType Version Access License
Effects of nitrogeno(725KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Yun]'s Articles
[Chen, Tiantian]'s Articles
[Song, Shuqun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Yun]'s Articles
[Chen, Tiantian]'s Articles
[Song, Shuqun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Yun]'s Articles
[Chen, Tiantian]'s Articles
[Song, Shuqun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Effects of nitrogenous nutrition on growth and nitrogen assimilation enzymes of dinoflagellate Akashiwo sanguinea.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.