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题名: Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT)
作者: Xie ShiJun(谢世筠)1,2; Zhang, Xiaojun1; Zhang, Jiquan1; Li, Fuhua1; Xiang, JianHai(相建海)1
刊名: PLOS ONE
出版日期: 2015-12-21
卷号: 10, 期号:12, 页码:0144922
DOI: 10.1371/journal.pone.0144922
文章类型: Article
英文摘要: White spot syndrome virus (WSSV) is a major pathogen in shrimp cultures. The interactions between viral proteins and their receptors on the surface of cells in a frontier target tissue are crucial for triggering an infection. In this study, a yeast two-hybrid (Y2H) library was constructed using cDNA obtained from the stomach and gut of Litopenaeus vannamei, to ascertain the role of envelope proteins in WSSV infection. For this purpose, VP37 was used as the bait in the Y2H library screening. Forty positive clones were detected after screening. The positive clones were analyzed and discriminated, and two clones belonging to the peritrophin family were subsequently confirmed as genuine positive clones. Sequence analysis revealed that both clones could be considered as the same gene, LV-peritrophin (LvPT). Co-immunoprecipitation confirmed the interaction between LvPT and VP37. Further studies in the Y2H system revealed that LvPT could also interact with other WSSV envelope proteins such as VP32, VP38A, VP39B, and VP41A. The distribution of LvPT in tissues revealed that LvPT was mainly expressed in the stomach than in other tissues. In addition, LvPT was found to be a secretory protein, and its chitin-binding ability was also confirmed.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000367092300026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.qdio.ac.cn/handle/337002/86652
Appears in Collections:实验海洋生物学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Xie, Shijun,Zhang, Xiaojun,Zhang, Jiquan,et al. Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT)[J]. PLOS ONE,2015,10(12):0144922.
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文件名: Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact.PDF
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