IOCAS-IR  > 实验海洋生物学重点实验室
Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT)
Xie, Shijun1,2; Zhang, Xiaojun1; Zhang, Jiquan1; Li, Fuhua1; Xiang, Jianhai1
2015-12-21
发表期刊PLOS ONE
卷号10期号:12页码:0144922
文章类型Article
摘要White spot syndrome virus (WSSV) is a major pathogen in shrimp cultures. The interactions between viral proteins and their receptors on the surface of cells in a frontier target tissue are crucial for triggering an infection. In this study, a yeast two-hybrid (Y2H) library was constructed using cDNA obtained from the stomach and gut of Litopenaeus vannamei, to ascertain the role of envelope proteins in WSSV infection. For this purpose, VP37 was used as the bait in the Y2H library screening. Forty positive clones were detected after screening. The positive clones were analyzed and discriminated, and two clones belonging to the peritrophin family were subsequently confirmed as genuine positive clones. Sequence analysis revealed that both clones could be considered as the same gene, LV-peritrophin (LvPT). Co-immunoprecipitation confirmed the interaction between LvPT and VP37. Further studies in the Y2H system revealed that LvPT could also interact with other WSSV envelope proteins such as VP32, VP38A, VP39B, and VP41A. The distribution of LvPT in tissues revealed that LvPT was mainly expressed in the stomach than in other tissues. In addition, LvPT was found to be a secretory protein, and its chitin-binding ability was also confirmed.
DOI10.1371/journal.pone.0144922
收录类别SCI
语种英语
WOS记录号WOS:000367092300026
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/86652
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Xie, Shijun,Zhang, Xiaojun,Zhang, Jiquan,et al. Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT)[J]. PLOS ONE,2015,10(12):0144922.
APA Xie, Shijun,Zhang, Xiaojun,Zhang, Jiquan,Li, Fuhua,&Xiang, Jianhai.(2015).Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).PLOS ONE,10(12),0144922.
MLA Xie, Shijun,et al."Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT)".PLOS ONE 10.12(2015):0144922.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Envelope Proteins of(6689KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xie, Shijun]的文章
[Zhang, Xiaojun]的文章
[Zhang, Jiquan]的文章
百度学术
百度学术中相似的文章
[Xie, Shijun]的文章
[Zhang, Xiaojun]的文章
[Zhang, Jiquan]的文章
必应学术
必应学术中相似的文章
[Xie, Shijun]的文章
[Zhang, Xiaojun]的文章
[Zhang, Jiquan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact.PDF
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。